DNA:RNA hybrids form at DNA double-strand breaks in transcriptionally active loci.
Journal
Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092
Informations de publication
Date de publication:
24 04 2020
24 04 2020
Historique:
received:
26
02
2020
accepted:
02
04
2020
revised:
24
03
2020
entrez:
26
4
2020
pubmed:
26
4
2020
medline:
1
4
2021
Statut:
epublish
Résumé
The recent discovery of DNA:RNA hybrids, or R-loops, actively forming at DNA double-strand breaks (DSBs) has unlocked fresh insight into how RNA participates in DNA repair. However, the manner of DSB-induced R-loop formation is vital in determining its mechanism of action and is currently under debate. Here, we analyse published DNA:RNA-hybrid sequencing to elucidate the features that determine DSB-induced R-loop formation. We found that pre-existing transcriptional activity was critical for R-loop generation at break sites, suggesting that these RNAs are transcribed prior to break induction. In addition, this appeared to be a specific DSB response at the break, distinct from traditional, co-transcriptionally formed R-loops. We hypothesise that R-loop formation is orchestrated by the damage response at transcriptionally active DSB loci to specifically maintain these genomic regions. Further investigation is required to fully understand how canonical repair processes regulate R-loops at breaks and how they participate in the repair process.
Identifiants
pubmed: 32332801
doi: 10.1038/s41419-020-2464-6
pii: 10.1038/s41419-020-2464-6
pmc: PMC7181826
doi:
Substances chimiques
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
280Subventions
Organisme : Medical Research Council
ID : MC_UP_A600_1024
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00025/6
Pays : United Kingdom
Organisme : Cancer Research UK
ID : A17196
Pays : United Kingdom
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