A loop-mediated isothermal amplification (LAMP) based assay for the rapid and sensitive group-specific detection of fumonisin producing Fusarium spp.
Animals
DNA, Fungal
/ genetics
Fumonisins
/ metabolism
Fusarium
/ genetics
Horse Diseases
/ microbiology
Horses
Humans
Molecular Diagnostic Techniques
/ methods
Mycotoxins
/ metabolism
Nucleic Acid Amplification Techniques
/ methods
Polyketide Synthases
/ genetics
Swine
Swine Diseases
/ microbiology
Zea mays
/ microbiology
Diagnosis
FFSC
Gene detection
Mycotoxin
fum1
Journal
International journal of food microbiology
ISSN: 1879-3460
Titre abrégé: Int J Food Microbiol
Pays: Netherlands
ID NLM: 8412849
Informations de publication
Date de publication:
16 Jul 2020
16 Jul 2020
Historique:
received:
22
08
2019
revised:
01
04
2020
accepted:
10
04
2020
pubmed:
26
4
2020
medline:
23
7
2020
entrez:
26
4
2020
Statut:
ppublish
Résumé
Fumonisins are mycotoxins that contaminate maize and maize-based food products, and feed. They have been associated with nerve system disorders in horses, pulmonary edema in swine as well as neural tube defects and esophageal cancer in humans. The fum1 gene codes for a polyketide synthase involved in the biosynthesis of fumonisins. It is present in the genomes of all fumonisin producing Fusarium spp. Reliable detection of fum1 can provide an estimate of the toxicological potential of cultures and food sources. Therefore, a fum1 specific LAMP assay was developed and tested with purified DNA of 48 different species from the Fusarium fujikuroi species complex (FFSC). The fum1 gene was detected in 22 species among which F. fujikuroi, F. globosum, F. nygamai, F. proliferatum, F. subglutinans and F. verticillioides were the most prominent fumonisin producers. None out of 92 tested non-Fusarium species showed cross reactions with the new assay. The lowest limit of detection (LOD) was 5 pg of genomic DNA per reaction for F. fujikuroi, F. nygamai and F. verticillioides. Higher LODs were found for other LAMP positive species. Apart from pure genomic DNA, the LAMP assay detected fumonisin-producers when 10
Identifiants
pubmed: 32334331
pii: S0168-1605(20)30121-5
doi: 10.1016/j.ijfoodmicro.2020.108627
pii:
doi:
Substances chimiques
DNA, Fungal
0
Fumonisins
0
Mycotoxins
0
Polyketide Synthases
79956-01-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108627Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.