Developing a novel resorptive hydroxyapatite-based bone substitute for over-critical size defect reconstruction: physicochemical and biological characterization and proof of concept in segmental rabbit's ulna reconstruction.
Animals
Biocompatible Materials
/ chemistry
Biomimetics
Bone Regeneration
/ physiology
Bone Substitutes
/ chemistry
Durapatite
/ chemistry
Osteogenesis
/ physiology
Polylactic Acid-Polyglycolic Acid Copolymer
/ chemistry
Printing, Three-Dimensional
Rabbits
Tissue Engineering
/ methods
Ulna
/ chemistry
3D printing
bone reconstruction
personalized medicine
tissue engineering
Journal
Biomedizinische Technik. Biomedical engineering
ISSN: 1862-278X
Titre abrégé: Biomed Tech (Berl)
Pays: Germany
ID NLM: 1262533
Informations de publication
Date de publication:
27 Aug 2020
27 Aug 2020
Historique:
received:
20
08
2019
accepted:
09
12
2019
pubmed:
27
4
2020
medline:
7
4
2021
entrez:
27
4
2020
Statut:
ppublish
Résumé
The aim of this study was to develop novel hydroxyapatite (HAP)-based bioactive bone replacement materials for segmental osteotomy reconstruction. Customized three-dimensional (3D) bone construct was manufactured from nanohydroxyapatite (nHAP) with poly(lactide-co-glycolide) (PLGA) coating using 3D models derived from the computed tomography (CT) scanning of the rabbit's ulna and gradient 3D printing of the bone substitute mimicking the anatomical shape of the natural bone defect. Engineered construct revealed adequate micro-architectural design for successful bone regeneration having a total porosity of 64% and an average pore size of 256 μm. Radiography and micro-CT analysis depicted new bone apposition through the whole length of the reconstructed ulna with a small area of non-resorbed construct in the central area of defect. Histological analysis revealed new bone formation with both endochondral and endesmal type of ossification. Immunohistochemistry analysis depicted the presence of bone formation indicators - bone morphogenetic protein (BMP), osteocalcin (OCN) and osteopontin (OPN) within newly formed bone. Manufactured personalized construct acts as a "smart" responsive biomaterial capable of modulating the functionality and potential for the personalized bone reconstruction on a clinically relevant length scale.
Identifiants
pubmed: 32335536
doi: 10.1515/bmt-2019-0218
pii: bmt-2019-0218
doi:
Substances chimiques
Biocompatible Materials
0
Bone Substitutes
0
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
491-505Commentaires et corrections
Type : ErratumIn
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