Antidiabetic drug metformin affects the developmental competence of cleavage-stage embryos.
Adult
Animals
Blastocyst
/ drug effects
Blood Glucose
/ drug effects
Diabetes, Gestational
/ blood
Disease Models, Animal
Embryonic Development
/ drug effects
Female
Fertilization in Vitro
/ trends
Humans
Hypoglycemic Agents
/ adverse effects
Insulin
/ metabolism
Insulin Resistance
/ genetics
Metformin
/ adverse effects
Mice
Polycystic Ovary Syndrome
/ blood
Pregnancy
Blastocyst rate
In vitro maturation
Metformin
Polycystic ovarian syndrome
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
15
10
2019
accepted:
30
01
2020
pubmed:
27
4
2020
medline:
14
1
2021
entrez:
27
4
2020
Statut:
ppublish
Résumé
Metformin is the most commonly prescribed drug in the management of metabolic disorders such as polycystic ovarian syndrome (PCOS) and gestational diabetes in women of reproductive age. Insulin-sensitizing effect of metformin helps in improving from PCOS features such as hyperandrogenism, anovulation, and infertility. However, its ability to cross placental barrier raises concern about safety of the drug on early embryonic development. In this study, we evaluated the effect of metformin on the ovarian function and embryo development. Adult Swiss albino female mice were administered with metformin (0, 50, 100, and 200 mg/kg body weight) for 4 weeks and assessed for reproductive function and preimplantation embryo development. Further, effect of metformin (0, 10, 25, 50, 100, 250, and 500 μg/mL) exposure to 2-cell-stage embryos was tested under in vitro conditions. Metformin did not alter the body weight, blood glucose, ovarian weight, and follicular reserve. However, the early embryo development was significantly affected in mice treated with metformin in vivo at highest dose. Moreover, embryos which were exposed to metformin in vitro showed dose-dependent decline in blastocyst rate and hatching rate. Furthermore, at highest concentration of metformin (500 μg/mL), all the embryos were arrested at compaction stage. The study revealed that metformin affects the early embryonic development and raises concern about its use during conception.
Identifiants
pubmed: 32335799
doi: 10.1007/s10815-020-01709-6
pii: 10.1007/s10815-020-01709-6
pmc: PMC7244706
doi:
Substances chimiques
Blood Glucose
0
Hypoglycemic Agents
0
Insulin
0
Metformin
9100L32L2N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1227-1238Subventions
Organisme : Science and Engineering Research Board (SERB), Department of Science and Technology, Govt. of India
ID : Grant. No: EMR/2016/002077/2017
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