Molecular characterization of Brucella ceti from a bottlenose dolphin (Tursiops truncatus) with osteomyelitis in the western Pacific.


Journal

The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360

Informations de publication

Date de publication:
24 Jun 2020
Historique:
pubmed: 28 4 2020
medline: 27 2 2021
entrez: 28 4 2020
Statut: ppublish

Résumé

Although the presence of Brucella spp. in the western Pacific has been suggested by epidemiological studies on cetaceans, it has not been confirmed by bacterial isolation. Here, for the first time, we report that a marine Brucella strain was isolated in the western Pacific from a bottlenose dolphin with osteomyelitis. The isolate from the lesion was confirmed to be B. ceti of sequence type 27 by multilocus sequence typing and Bruce-ladder PCR. Infrequent-restriction-site PCR and omp2 gene sequencing revealed that molecular characteristics of this isolate were similar to those of Brucella DNA previously detected from minke whales in the western North Pacific. These results suggest that genetically related Brucella strains circulate in cetacean species in this region.

Identifiants

pubmed: 32336742
doi: 10.1292/jvms.20-0015
pmc: PMC7324819
doi:

Substances chimiques

DNA, Bacterial 0

Types de publication

Case Reports Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

754-758

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Auteurs

Yuichi Ueno (Y)

Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.

Makio Yanagisawa (M)

Okinawa Churashima Foundation, 888 Ishikawa, Kunigamigun Motobucho, Okinawa 905-0214, Japan.

Sayuri Kino (S)

Okinawa Churashima Foundation, 888 Ishikawa, Kunigamigun Motobucho, Okinawa 905-0214, Japan.

Satoru Shigeno (S)

Okinawa Prefectural Institute of Animal Health, 3-1 Kenekadan, Uruma, Okinawa 904-2241, Japan.

Makoto Osaki (M)

Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.

Daisuke Takamatsu (D)

Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagito, Gifu, Gifu 501-1193, Japan.

Ken Katsuda (K)

Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.

Tadashi Maruyama (T)

School of Marine Biosciences, Kitasato University, 1-15-1 Kitasato, Minami Ward, Sagamihara, Kanagawa 252-0373, Japan.

Kazue Ohishi (K)

School of Engineering, Tokyo Polytechnic University, 1583 Iiyama, Atsugi, Kanagawa 243-0297, Japan.

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Classifications MeSH