Direct inhibition of arcuate kisspeptin neurones by neuropeptide Y in the male and female mouse.
Agouti-Related Protein
/ metabolism
Animals
Arcuate Nucleus of Hypothalamus
/ metabolism
Calcium
/ metabolism
Female
Gonadotropin-Releasing Hormone
/ metabolism
Kisspeptins
/ metabolism
Luteinizing Hormone
/ metabolism
Male
Mice
Neurokinin B
/ metabolism
Neuropeptide Y
/ metabolism
alpha-MSH
/ pharmacology
GCaMP
GnRH
NPY receptor
kisspeptin
neuropeptide Y
Journal
Journal of neuroendocrinology
ISSN: 1365-2826
Titre abrégé: J Neuroendocrinol
Pays: United States
ID NLM: 8913461
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
12
02
2020
revised:
13
03
2020
accepted:
28
03
2020
pubmed:
28
4
2020
medline:
31
5
2022
entrez:
28
4
2020
Statut:
ppublish
Résumé
Adverse energy states exert a potent suppressive influence on the reproductive axis by inhibiting the pulsatile release of gonadotrophin-releasing hormone and luteinising hormone. One potential mechanism underlying this involves the metabolic-sensing pro-opiomelanocortin and agouti-related peptide/neuropeptide Y (AgRP/NPY) neuronal populations directly controlling the activity of the arcuate nucleus kisspeptin neurones comprising the gonadotrophin-releasing hormone pulse generator. Using acute brain slice electrophysiology and calcium imaging approaches in Kiss1-GFP and Kiss1-GCaMP6 mice, we investigated whether NPY and α-melanocyte-stimulating hormone provide a direct modulatory influence on the activity of arcuate kisspeptin neurones in the adult mouse. NPY was found to exert a potent suppressive influence upon the neurokinin B-evoked firing of approximately one-half of arcuate kisspeptin neurones in both sexes. This effect was blocked partially by the NPY1R antagonist BIBO 3304, whereas the NPY5R antagonist L152,804 was ineffective. NPY also suppressed the neurokinin B-evoked increase in intracellular calcium levels in the presence of tetrodotoxin and amino acid receptor antagonists, indicating that the inhibitory effects of NPY are direct on kisspeptin neurones. By contrast, no effects of α-melanocyte-stimulating hormone were found on the excitability of arcuate kisspeptin neurones. These studies provide further evidence supporting the hypothesis that AgRP/NPY neurones link energy status and luteinising hormone pulsatility by demonstrating that NPY has a direct suppressive influence upon the activity of a subpopulation of arcuate kisspeptin neurones.
Substances chimiques
Agouti-Related Protein
0
Kisspeptins
0
Neuropeptide Y
0
Gonadotropin-Releasing Hormone
33515-09-2
alpha-MSH
581-05-5
Neurokinin B
86933-75-7
Luteinizing Hormone
9002-67-9
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12849Subventions
Organisme : New Zealand Health Research Council
Informations de copyright
© 2020 British Society for Neuroendocrinology.
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