SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase.

Animals DEAD-box RNA Helicases / genetics DNA Helicases G-Quadruplexes Mice RNA, Long Noncoding / genetics RNA, Messenger / genetics
G-quadruplex Myogenesis RNA helicases RNA-RNA interactions lncRNAs

Journal

EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049

Informations de publication

Date de publication:
04 06 2020
Historique:
received: 23 12 2019
revised: 26 03 2020
accepted: 31 03 2020
pubmed: 28 4 2020
medline: 28 4 2021
entrez: 28 4 2020
Statut: ppublish

Résumé

Guanine-quadruplexes (G4) included in RNA molecules exert several functions in controlling gene expression at post-transcriptional level; however, the molecular mechanisms of G4-mediated regulation are still poorly understood. Here, we describe a regulatory circuitry operating in the early phases of murine muscle differentiation in which a long non-coding RNA (SMaRT) base pairs with a G4-containing mRNA (Mlx-γ) and represses its translation by counteracting the activity of the DHX36 RNA helicase. The time-restricted, specific effect of lnc-SMaRT on the translation of Mlx-γ isoform modulates the general subcellular localization of total MLX proteins, impacting on their transcriptional output and promoting proper myogenesis and mature myotube formation. Therefore, the circuitry made of lnc-SMaRT, Mlx-γ, and DHX36 not only plays an important role in the control of myogenesis but also unravels a molecular mechanism where G4 structures and G4 unwinding activities are regulated in living cells.

Identifiants

DOI: 10.15252/embr.201949942 PMID: 32337838 PMC: PMC7271651
pubmed: 32337838
doi: 10.15252/embr.201949942
pmc: PMC7271651
doi:

Substances chimiques

RNA, Long Noncoding 0
RNA, Messenger 0
Dhx36 protein, mouse EC 3.6.1.-
DNA Helicases EC 3.6.4.-
DEAD-box RNA Helicases EC 3.6.4.13

Banques de données

GEO
['GSE128486']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e49942

Subventions

Organisme : ERC - European Research Council 2013
ID : 340172-MUNCODD
Pays : International
Organisme : EC - FP7 Marie Curie
ID : ITN - 2013 GA 607720
Pays : International
Organisme : Fondazione Telethon (Telethon Foundation)
ID : GGP16213
Pays : International
Organisme : Associazione Italiana per la Ricerca sul Cancro (AIRC)
ID : IG 2019 Id. 23053
Pays : International
Organisme : Horizon 2020
ID : ERC-2019-SyG (855923-ASTRA)
Pays : International
Organisme : MIUR - Ministero dell'Istruzione, Università e Ricerca
ID : PRIN-2017 (2017P352Z4)
Pays : International

Informations de copyright

© 2020 The Authors. Published under the terms of the CC BY 4.0 license.

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Auteurs

Julie Martone (J)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.

Davide Mariani (D)

Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.

Tiziana Santini (T)

Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.

Adriano Setti (A)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.

Sama Shamloo (S)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.

Alessio Colantoni (A)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.

Francesca Capparelli (F)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.

Alessandro Paiardini (A)

Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy.

Dacia Dimartino (D)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.

Mariangela Morlando (M)

Department of Pharmaceutical Sciences, University of Perugia, Perugia, Italy.

Irene Bozzoni (I)

Department of Biology and Biotechnology, Charles Darwin, Sapienza University of Rome, Rome, Italy.
Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, Italy.
ORCID: 0000-0002-3485-8537

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › SMaRT lncRNA controls translation of a...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › RNA helicases › DEAD-box RNA helicases › SMaRT lncRNA controls translation of a...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Isomerases › Helicase › SMaRT lncRNA controls translation of a...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › G-quadruplexes › SMaRT lncRNA controls translation of a...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › SMaRT lncRNA controls translation of a...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › ARN long non codant › SMaRT lncRNA controls translation of a...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › SMaRT lncRNA controls translation of a...