Analyzing the impact of Mycobacterium tuberculosis infection on primary human macrophages by combined exploratory and targeted metabolomics.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
27 04 2020
27 04 2020
Historique:
received:
29
11
2019
accepted:
17
03
2020
entrez:
29
4
2020
pubmed:
29
4
2020
medline:
26
11
2020
Statut:
epublish
Résumé
The pathogenic success of Mycobacterium tuberculosis (Mtb) is tightly linked to its ability to recalibrate host metabolic processes in infected host macrophages. Since changes in cellular metabolic intermediates or pathways also affect macrophage function in response to pathogens, we sought to analyse specific metabolic alterations induced by Mtb infection. Stimulation of macrophages with Mtb lysate or lipopolysaccharide (LPS) induced a relative increase in glycolysis versus oxidative phosphorylation. Cellular metabolomics revealed that Mtb infection induced a distinct metabolic profile compared to LPS in both M1 and M2 macrophages. Specifically, Mtb infection resulted in elevated intracellular levels of nicotinamide adenine dinucleotide (NAD
Identifiants
pubmed: 32341411
doi: 10.1038/s41598-020-62911-1
pii: 10.1038/s41598-020-62911-1
pmc: PMC7184630
doi:
Substances chimiques
Lipopolysaccharides
0
Glutamine
0RH81L854J
Glucose
IY9XDZ35W2
Creatine
MU72812GK0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7085Références
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