The effects of intro-oral parathyroid hormone on the healing of tooth extraction socket: an experimental study on hyperglycemic rats.
Animals
Blood Glucose
/ analysis
Calcium
/ blood
Calcium-Regulating Hormones and Agents
/ pharmacology
Diabetes Mellitus, Experimental
/ physiopathology
Hydrogels
Male
Osteogenesis
/ drug effects
Parathyroid Hormone
/ pharmacology
Random Allocation
Rats, Sprague-Dawley
Reproducibility of Results
Surgical Wound
/ drug therapy
Time Factors
Tooth Extraction
/ methods
Tooth Socket
/ diagnostic imaging
Treatment Outcome
Wound Healing
/ drug effects
X-Ray Microtomography
Journal
Journal of applied oral science : revista FOB
ISSN: 1678-7765
Titre abrégé: J Appl Oral Sci
Pays: Brazil
ID NLM: 101189774
Informations de publication
Date de publication:
2020
2020
Historique:
received:
20
11
2019
accepted:
24
02
2020
entrez:
30
4
2020
pubmed:
30
4
2020
medline:
20
5
2020
Statut:
ppublish
Résumé
Objective To investigate the effects of intro-oral injection of parathyroid hormone (PTH) on tooth extraction wound healing in hyperglycemic rats. Methodology 60 male Sprague-Dawley rats were randomly divided into the normal group (n=30) and DM group (n=30). Type 1 diabetes mellitus (DM) was induced by streptozotocin. After extracting the left first molar of all rats, each group was further divided into 3 subgroups (n=10 per subgroup), receiving the administration of intermittent PTH, continuous PTH and saline (control), respectively. The intermittent-PTH group received intra-oral injection of PTH three times per week for two weeks. A thermosensitive controlled-release hydrogel was synthesized for continuous-PTH administration. The serum chemistry was determined to evaluate the systemic condition. All animals were sacrificed after 14 days. Micro-computed tomography (Micro-CT) and histological analyses were used to evaluate the healing of extraction sockets. Results The level of serum glucose in the DM groups was significantly higher than that in the non-DM groups (p<0.05); the level of serum calcium was similar in all groups (p>0.05). Micro-CT analysis showed that the DM group had a significantly lower alveolar bone trabecular number (Tb.N) and higher trabecular separation (Tb.Sp) than the normal group (p<0.05). The histological analyses showed that no significant difference in the amount of new bone (hard tissue) formation was found between the PTH and non-PTH groups (p>0.05). Conclusions Bone formation in the extraction socket of the type 1 diabetic rats was reduced. PTH did not improve the healing of hard and soft tissues. The different PTH administration regimes (continuous vs. intermittent) had similar effect on tissue healing. These results demonstrated that the metabolic characteristics of the hyperglycemic rats produced a condition that was unable to respond to PTH treatment.
Identifiants
pubmed: 32348445
pii: S1678-77572020000100442
doi: 10.1590/1678-7757-2019-0690
pmc: PMC7185986
pii:
doi:
Substances chimiques
Blood Glucose
0
Calcium-Regulating Hormones and Agents
0
Hydrogels
0
Parathyroid Hormone
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e20190690Références
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