β-ecdysone promotes osteogenic differentiation of bone marrow mesenchymal stem cells.
Animals
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Ecdysterone
/ pharmacology
Femur Head
/ cytology
Humans
Lipopolysaccharides
/ pharmacology
Mesenchymal Stem Cell Transplantation
Mesenchymal Stem Cells
/ cytology
Methylprednisolone
/ pharmacology
Osteogenesis
/ drug effects
Rats
BMSCs
osteonecrosis of the femoral head, PI3K/Akt
βEcd
Journal
The journal of gene medicine
ISSN: 1521-2254
Titre abrégé: J Gene Med
Pays: England
ID NLM: 9815764
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
10
03
2020
revised:
13
04
2020
accepted:
13
04
2020
pubmed:
1
5
2020
medline:
24
8
2021
entrez:
1
5
2020
Statut:
ppublish
Résumé
β-ecdysone (βEcd) has numerous pharmacological effects, although its role in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) has not yet been explored. In cell experiments, BMSCs were induced to differentiate by osteogenic induction medium (OIM) or βEcd. In animal experiments, an osteonecrosis of the femoral head (ONFH) rat model was established using lipopolysaccharide plus methylprednisolone and treating the rats with βEcd. The osteogenic differentiation capacity of human BMSCs (hBMSCs) was analyzed by alkaline phosphatase and alizarin red S staining. Histopathological changes in rat femoral head tissues were observed by hematoxylin and eosin staining. The expression levels of RUNX2, COL1A1, OCN and phosphorylated Akt in BMSCs from rat femoral head tissues were measured by a quantitative real-time polymerase chain reaction or western blot analysis. Alkaline phosphatase activity and calcium nodules in the βEcd-treated BMSC group dose-dependently increased compared to those in the control and OIM groups. The hematoxylin and eosin staining results indicated that femoral head tissues of ONFH rats showed typical osteonecrosis, which could be ameliorated by βEcd. Western blot, quantitative real-time polymerase chain reaction and immunohistochemistry assays demonstrated that the expression levels of RUNX2, COL1A1 and OCN in hBMSCs and femoral head tissue models were obviously increased after βEcd treatment, and phosphoinositide 3-kinase and Akt phosphorylation were also increased. βEcd may be beneficial for the recovery of ONFH patients by accelerating osteogenic differentiation of BMSCs, which may be a novel therapy for related diseases.
Sections du résumé
BACKGROUND
β-ecdysone (βEcd) has numerous pharmacological effects, although its role in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) has not yet been explored.
METHODS
In cell experiments, BMSCs were induced to differentiate by osteogenic induction medium (OIM) or βEcd. In animal experiments, an osteonecrosis of the femoral head (ONFH) rat model was established using lipopolysaccharide plus methylprednisolone and treating the rats with βEcd. The osteogenic differentiation capacity of human BMSCs (hBMSCs) was analyzed by alkaline phosphatase and alizarin red S staining. Histopathological changes in rat femoral head tissues were observed by hematoxylin and eosin staining. The expression levels of RUNX2, COL1A1, OCN and phosphorylated Akt in BMSCs from rat femoral head tissues were measured by a quantitative real-time polymerase chain reaction or western blot analysis.
RESULTS
Alkaline phosphatase activity and calcium nodules in the βEcd-treated BMSC group dose-dependently increased compared to those in the control and OIM groups. The hematoxylin and eosin staining results indicated that femoral head tissues of ONFH rats showed typical osteonecrosis, which could be ameliorated by βEcd. Western blot, quantitative real-time polymerase chain reaction and immunohistochemistry assays demonstrated that the expression levels of RUNX2, COL1A1 and OCN in hBMSCs and femoral head tissue models were obviously increased after βEcd treatment, and phosphoinositide 3-kinase and Akt phosphorylation were also increased.
CONCLUSIONS
βEcd may be beneficial for the recovery of ONFH patients by accelerating osteogenic differentiation of BMSCs, which may be a novel therapy for related diseases.
Substances chimiques
Lipopolysaccharides
0
Ecdysterone
5289-74-7
Methylprednisolone
X4W7ZR7023
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3207Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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