Discovery of seven novel mutations of gyrB, parC and parE in Salmonella Typhi and Paratyphi strains from Jiangsu Province of China.
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
China
Ciprofloxacin
/ pharmacology
DNA Gyrase
/ genetics
Drug Resistance, Bacterial
/ genetics
Microbial Sensitivity Tests
Mutation
/ genetics
Nalidixic Acid
/ pharmacology
Quinolones
/ pharmacology
Salmonella paratyphi A
/ drug effects
Salmonella typhi
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 04 2020
30 04 2020
Historique:
received:
22
11
2019
accepted:
15
04
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
15
12
2020
Statut:
epublish
Résumé
To investigate the prevalence of Salmonella Typhi and Paratyphi resistance to quinolones and characterize the underlying mechanism in Jiangsu Province of China. Antimicrobial susceptibility testing was performed using Kirby-Bauer disc diffusion system. Quinolone resistance-determining region (QRDR), plasmid-mediated quinolone resistance (PMQR) determinant genes were detected by PCR and sequencing. Out of 239 Salmonella isolates, 164 were S. Typhi and 75 were S. Paratyphi. 128 (53.6%) Salmonella isolates were resistant to nalidixic acid; 11 (4.6%) isolates to ciprofloxacin and 66 (27.6%) isolates were intermediate to ciprofloxacin. QRDR were present in 69 S. Typhi isolates, among which mutation at codon 83 (n = 45) and 133 (n = 61) predominated. In S. Paratyphi, the most common mutations were detected in gyrA at codon 83(n = 24) and parC: T57S (n = 8). Seven mutations were first reported in Salmonella isolates including gyrB: S426G, parC: D79G and parE: [S498T, E543K, V560G, I444S, Y434S]. PMQR genes including qnrD1, qnrA1, qnrB4, aac (6')-Ib-cr4 and qnrS1 were detected in 1, 2, 3, 7 and 9 isolates, relatively. High resistance to quinolones in Salmonella remains a serious problem in Jiangsu, China. The presence of the novel mutations increases the complexity of quinolone-resistant genotypes and poses a threat to public health. Subject terms: Salmonella Typhi, Salmonella Paratyphi, antimicrobial resistance, QRDR, PMQR.
Identifiants
pubmed: 32355184
doi: 10.1038/s41598-020-64346-0
pii: 10.1038/s41598-020-64346-0
pmc: PMC7193621
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Quinolones
0
Nalidixic Acid
3B91HWA56M
Ciprofloxacin
5E8K9I0O4U
DNA Gyrase
EC 5.99.1.3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7359Références
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