Genetic diversity and ecology of coronaviruses hosted by cave-dwelling bats in Gabon.
Alphacoronavirus
/ genetics
Animals
Base Sequence
/ genetics
Betacoronavirus
/ genetics
Caves
Chiroptera
/ virology
Coronavirus 229E, Human
/ genetics
Coronavirus Infections
/ epidemiology
Eulipotyphla
/ virology
Gabon
/ epidemiology
Genetic Variation
Humans
Phylogeny
Prevalence
Primates
/ genetics
RNA, Viral
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
Rodentia
/ genetics
Seasons
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 04 2020
30 04 2020
Historique:
received:
17
09
2018
accepted:
06
04
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
22
9
2020
Statut:
epublish
Résumé
Little research on coronaviruses has been conducted on wild animals in Africa. Here, we screened a wide range of wild animals collected in six provinces and five caves of Gabon between 2009 and 2015. We collected a total of 1867 animal samples (cave-dwelling bats, rodents, non-human primates and other wild animals). We explored the diversity of CoVs and determined the factors driving the infection of CoVs in wild animals. Based on a nested reverse transcription-polymerase chain reaction, only bats, belonging to the Hipposideros gigas (4/156), Hipposideros cf. ruber (13/262) and Miniopterus inflatus (1/249) species, were found infected with CoVs. We identified alphacoronaviruses in H. gigas and H. cf. ruber and betacoronaviruses in H. gigas. All Alphacoronavirus sequences grouped with Human coronavirus 229E (HCoV-229E). Ecological analyses revealed that CoV infection was significantly found in July and October in H. gigas and in October and November in H. cf ruber. The prevalence in the Faucon cave was significantly higher. Our findings suggest that insectivorous bats harbor potentially zoonotic CoVs; highlight a probable seasonality of the infection in cave-dwelling bats from the North-East of Gabon and pointed to an association between the disturbance of the bats' habitat by human activities and CoV infection.
Identifiants
pubmed: 32355260
doi: 10.1038/s41598-020-64159-1
pii: 10.1038/s41598-020-64159-1
pmc: PMC7192909
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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