Glypican-3 electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids peroxidase-like catalytic silver deposition.
Aptamers, Nucleotide
/ chemistry
Biosensing Techniques
/ methods
Catalysis
Electrochemical Techniques
/ methods
Glypicans
/ blood
Graphite
/ chemistry
Hemin
/ chemistry
Humans
Hydrogen Peroxide
/ chemistry
Limit of Detection
Metal Nanoparticles
/ chemistry
Oxidation-Reduction
Reproducibility of Results
Silver
/ chemistry
Aptamer
Electrochemical nanobiosensor
Glypican-3
Hemin/graphene nanohybrids
Peroxidase-like catalytic silver deposition
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
30 04 2020
30 04 2020
Historique:
received:
28
10
2019
accepted:
19
04
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
2
6
2021
Statut:
epublish
Résumé
A Glypican-3 (GPC3) electrochemical aptamer nanobiosensor based on hemin/graphene nanohybrids (HGNs) peroxidase-like catalytic silver deposition and GPC3 aptamer has been constructed for the determination of GPC3. The HGNs were prepared by an one-step reduction method. Fourier transform infrared spectroscopy (FT-IR), ultraviolet spectroscopy (UV-vis), and transmission electron microscopy (TEM) were used to study the structure and morphological characteristics of the HGNs. The GPC3 electrochemical aptamer nanobiosensor was constructed using HGNs-aptamer (HGNs-Apt) as the signal probe and GPC3 aptamer as the capture probe. With the help of the catalytic action of peroxidase-like properties of HGNs, H
Identifiants
pubmed: 32356075
doi: 10.1007/s00604-020-04284-w
pii: 10.1007/s00604-020-04284-w
doi:
Substances chimiques
Aptamers, Nucleotide
0
GPC3 protein, human
0
Glypicans
0
Silver
3M4G523W1G
Hemin
743LRP9S7N
Graphite
7782-42-5
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
305Subventions
Organisme : National Nature Science Foundation of China
ID : No. 81760534
Pays : International
Organisme : Graduate Research and Innovation Projects of Jiangsu Province
ID : 2018YJCX69
Pays : International
Organisme : National Natural Science Foundation of China
ID : 61941108
Pays : International
Organisme : Natural Science Foundation of Guangxi Province
ID : No. 2108GXNSFAA281231
Pays : International