Performance of laterally elongated pillar array columns in capillary electrochromatography mode.
CEC
Microfluidic chips
Pillar array columns
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
02
01
2020
revised:
22
04
2020
accepted:
23
04
2020
pubmed:
3
5
2020
medline:
23
3
2021
entrez:
3
5
2020
Statut:
ppublish
Résumé
In the present study, cylindrical and laterally elongated pillar array columns were investigated for use in capillary electrochromatography. Minimal theoretical plate heights of H = 1.90 and 1.46 μm (in absence of sidewall effect) were obtained for coumarin C440 under unretained conditions for cylindrical and rectangular (laterally elongated, aspect ratio 4) pillar array columns, respectively. By comparing dispersion at the entire channel width to that at the central zone only, it appears that sidewall related dispersion significantly contributes to overall dispersion. A 40% reduction of the plate height was observed by taking into account only the central channel zone. A kinetic plot analysis was performed to evaluate the potential of the studied geometries by considering a maximum operating voltage of 20 kV as limiting parameter. It was demonstrated that rectangular radially elongated pillars produce a higher efficiency than cylindrical pillars and other microfabricated column structures for microchip capillary electrochromatography previously studied.
Identifiants
pubmed: 32358839
doi: 10.1002/elps.202000001
doi:
Substances chimiques
Coumarins
0
7-amino-4-methylcoumarin
OCY3JCT44X
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1287-1295Subventions
Organisme : European Research Council (ERC Starting Grant)
ID : 679033EVODIS ERC-2015-STG
Pays : International
Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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