Single-Cell Analysis Reveals Partial Reactivation of X Chromosome instead of Chromosome-wide Dampening in Naive Human Pluripotent Stem Cells.
X chromosome dampening
X chromosome inactivation
X chromosome upregulation
XIST
human embryos
human pluripotent stem cells
naive and primed pluripotency
single-cell RNA-seq
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
12 05 2020
12 05 2020
Historique:
received:
20
08
2019
revised:
31
03
2020
accepted:
31
03
2020
pubmed:
4
5
2020
medline:
17
4
2021
entrez:
4
5
2020
Statut:
ppublish
Résumé
Recently, a unique form of X chromosome dosage compensation has been demonstrated in human preimplantation embryos, which happens through the dampening of X-linked gene expression from both X chromosomes. Subsequently, X chromosome dampening has also been demonstrated in female human pluripotent stem cells (hPSCs) during the transition from primed to naive state. However, the existence of dampened X chromosomes in both embryos and hPSCs remains controversial. Specifically, in preimplantation embryos it has been shown that there is inactivation of X chromosome instead of dampening. Here, we performed allelic analysis of X-linked genes at the single-cell level in hPSCs and found that there is partial reactivation of the inactive X chromosome instead of chromosome-wide dampening upon conversion from primed to naive state. In addition, our analysis suggests that the reduced X-linked gene expression in naive hPSCs might be the consequence of erasure of active X chromosome upregulation.
Identifiants
pubmed: 32359444
pii: S2213-6711(20)30117-X
doi: 10.1016/j.stemcr.2020.03.027
pmc: PMC7221091
pii:
doi:
Substances chimiques
RNA, Long Noncoding
0
XIST non-coding RNA
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
745-754Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Références
Mol Biol Evol. 2016 Dec;33(12):3104-3107
pubmed: 27593371
Development. 2015 Sep 15;142(18):3090-9
pubmed: 26395138
Cell Stem Cell. 2009 Jun 5;4(6):487-92
pubmed: 19497275
Nature. 2011 Apr 21;472(7343):370-4
pubmed: 21471966
Nat Genet. 2010 Dec;42(12):1043-7
pubmed: 21102464
Nature. 1961 Apr 22;190:372-3
pubmed: 13764598
Dev Cell. 2013 Apr 15;25(1):55-68
pubmed: 23523075
Chromosoma. 2019 Jul 4;:
pubmed: 31273451
Dev Cell. 2017 Feb 6;40(3):289-301.e3
pubmed: 28132849
Nat Genet. 2011 Oct 23;43(12):1179-85
pubmed: 22019781
PLoS Biol. 2007 Dec;5(12):e326
pubmed: 18076287
Genome Biol. 2015 Aug 03;16:149
pubmed: 26235224
EMBO Rep. 2018 Aug;19(8):
pubmed: 30037898
Philos Trans R Soc Lond B Biol Sci. 2017 Nov 5;372(1733):
pubmed: 28947660
Sci Rep. 2017 Sep 7;7(1):10794
pubmed: 28883481
Cell. 2016 May 5;165(4):1012-26
pubmed: 27062923
Cell Stem Cell. 2017 Jan 5;20(1):87-101
pubmed: 27989770
Cell Stem Cell. 2016 Oct 6;19(4):502-515
pubmed: 27424783
Nat Struct Mol Biol. 2019 Oct;26(10):963-969
pubmed: 31582851
Nat Genet. 2006 Jan;38(1):47-53
pubmed: 16341221
Sci Rep. 2017 Jun 16;7(1):3729
pubmed: 28623283
Cell Stem Cell. 2014 Oct 2;15(4):471-487
pubmed: 25090446
Nat Genet. 2011 Nov 28;43(12):1169-70; author reply 1171-2
pubmed: 22120049