The role of macrophages in osseointegration of dental implants: An experimental study in vivo.
foreign body reaction
macrophage depletion
osseointegration
titanium implant
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
01 11 2020
01 11 2020
Historique:
received:
18
12
2019
revised:
26
03
2020
accepted:
04
04
2020
pubmed:
5
5
2020
medline:
9
11
2021
entrez:
5
5
2020
Statut:
ppublish
Résumé
To investigate the role of macrophages in the osseointegration of dental implants through induced macrophage reduction in a murine model. Fifty-four Sprague-Dawley rats with bilateral maxillary first molars replaced by titanium implants were randomly assigned into three groups. For the test group, macrophages were depleted by tail-vein injection of clodronate liposome (20 mg/kg) 3 days before implantation and reinjection every 3 days until the sacrifice of the rats (10 mg/kg). Animals treated with Phosphate Buffer saline (PBS) alone or empty liposome were included as controls. Samples contained implants were retrieved after 3, 7, 14, and 28 days, and the alterations of macrophages (CD68) and osteoblasts (Osterix) were evaluated using histology and immunohistochemistry technique. Histological analysis showed that new bone gradually formed within the lateral chamber regions in both the Control group and the Lip group, whereas bone healing was delayed at the first 2-weeks despite of pronounced newly formed peri-implant bone at 4 weeks in the Lipclod group. The bone-to-implant contact was significantly higher in the Lip and Control group than in the Lipclod group after 2 weeks. Immunohistochemical analysis showed that CD68+ cells were present both in the central region and in direct contact with implant surface throughout the healing period. Macrophages depletion reduced osteoblast amounts and new bone formation around implants in the first 2 weeks, and have no adverse impacts on the final formation of osseointegration. Macrophages play a dual role in both regulating the bone healing process and immune response to implant installation during the early stages.
Substances chimiques
Antigens, CD
0
Antigens, Differentiation, Myelomonocytic
0
CD68 protein, rat
0
Dental Implants
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2206-2216Informations de copyright
© 2020 Wiley Periodicals, Inc.
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