Salmonella Typhimurium discreet-invasion of the murine gut absorptive epithelium.
Animals
Bacterial Adhesion
Bacterial Proteins
/ genetics
Dogs
Guanine Nucleotide Exchange Factors
/ genetics
HeLa Cells
Humans
Intestinal Mucosa
/ metabolism
Madin Darby Canine Kidney Cells
Mice
Mice, Knockout
Microfilament Proteins
/ genetics
Salmonella Infections
/ genetics
Salmonella typhimurium
/ genetics
Type I Secretion Systems
/ genetics
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
18
01
2020
accepted:
26
03
2020
revised:
14
05
2020
pubmed:
5
5
2020
medline:
22
7
2020
entrez:
5
5
2020
Statut:
epublish
Résumé
Salmonella enterica serovar Typhimurium (S.Tm) infections of cultured cell lines have given rise to the ruffle model for epithelial cell invasion. According to this model, the Type-Three-Secretion-System-1 (TTSS-1) effectors SopB, SopE and SopE2 drive an explosive actin nucleation cascade, resulting in large lamellipodia- and filopodia-containing ruffles and cooperative S.Tm uptake. However, cell line experiments poorly recapitulate many of the cell and tissue features encountered in the host's gut mucosa. Here, we employed bacterial genetics and multiple imaging modalities to compare S.Tm invasion of cultured epithelial cell lines and the gut absorptive epithelium in vivo in mice. In contrast to the prevailing ruffle-model, we find that absorptive epithelial cell entry in the mouse gut occurs through "discreet-invasion". This distinct entry mode requires the conserved TTSS-1 effector SipA, involves modest elongation of local microvilli in the absence of expansive ruffles, and does not favor cooperative invasion. Discreet-invasion preferentially targets apicolateral hot spots at cell-cell junctions and shows strong dependence on local cell neighborhood. This proof-of-principle evidence challenges the current model for how S.Tm can enter gut absorptive epithelial cells in their intact in vivo context.
Identifiants
pubmed: 32365138
doi: 10.1371/journal.ppat.1008503
pii: PPATHOGENS-D-20-00095
pmc: PMC7224572
doi:
Substances chimiques
Bacterial Proteins
0
Guanine Nucleotide Exchange Factors
0
Microfilament Proteins
0
SipA protein, Salmonella
0
SopE protein, Salmonella
0
SopE2 protein, Salmonella typhimurium
0
Type I Secretion Systems
0
SopB protein, Salmonella
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008503Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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