A Fluorescence-Based Method to Measure ADP/ATP Exchange of Recombinant Adenine Nucleotide Translocase in Liposomes.

Adenine Nucleotide Translocator 1 / metabolism Adenosine Diphosphate / metabolism Adenosine Triphosphate / metabolism Animals Fluorescent Dyes Fluorometry / methods Glycine / analogs & derivatives Kinetics Magnesium / metabolism Mice Recombinant Proteins / metabolism Unilamellar Liposomes / metabolism Xanthenes
fluorescence magnesium greenTM fluorescent dye mitochondria model systems radioactivity recombinant adenine nucleotide translocase reconstitution into liposomes

Journal

Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414

Informations de publication

Date de publication:
29 04 2020
Historique:
received: 30 03 2020
revised: 24 04 2020
accepted: 27 04 2020
entrez: 6 5 2020
pubmed: 6 5 2020
medline: 7 4 2021
Statut: epublish

Résumé

Several mitochondrial proteins, such as adenine nucleotide translocase (ANT), aspartate/glutamate carrier, dicarboxylate carrier, and uncoupling proteins 2 and 3, are suggested to have dual transport functions. While the transport of charge (protons and anions) is characterized by an alteration in membrane conductance, investigating substrate transport is challenging. Currently, mainly radioactively labeled substrates are used, which are very expensive and require stringent precautions during their preparation and use. We present and evaluate a fluorescence-based method using Magnesium Green (MgGr

Identifiants

DOI: 10.3390/biom10050685 PMID: 32365477 PMC: PMC7277544
pubmed: 32365477
pii: biom10050685
doi: 10.3390/biom10050685
pmc: PMC7277544
pii:
doi:

Substances chimiques

Adenine Nucleotide Translocator 1 0
Fluorescent Dyes 0
Recombinant Proteins 0
Unilamellar Liposomes 0
Xanthenes 0
magnesium green 170516-41-3
Adenosine Diphosphate 61D2G4IYVH
Adenosine Triphosphate 8L70Q75FXE
Magnesium I38ZP9992A
Glycine TE7660XO1C

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Austrian Science Fund FWF
ID : P 31559
Pays : Austria
Organisme : Austrian Research Fund, FWF
ID : P31559 to EEP
Pays : International

Références

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Auteurs

Jürgen Kreiter (J)

Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
ORCID: 0000-0001-5877-762X

Eric Beitz (E)

Pharmaceutical and Medicinal Chemistry, Christian-Albrechts-University of Kiel, 24118 Kiel, Germany.
ORCID: 0000-0001-5912-6626

Elena E Pohl (EE)

Institute of Physiology, Pathophysiology and Biophysics, Department of Biomedical Sciences, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
ORCID: 0000-0002-0604-5950

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines mitochondriales › Protéines de transport de la membrane mitochondriale › Mitochondrial ADP, ATP Translocases › Translocateur-1 de nucléotides adényliques › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › ADP › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Ribonucléotides › Nucléotides adényliques › Adénosine triphosphate › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Eucaryotes › Animaux › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Photométrie › Mesures de luminescence › Fluorimétrie › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Glycine › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux légers › Magnésium › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomimétiques › Membrane artificielle › Liposomes › Liposomes unilamellaires › A Fluorescence-Based Method to Measure ADP/ATP...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques 3 noyaux › Xanthènes › A Fluorescence-Based Method to Measure ADP/ATP...