Evaluation of the Automated Indirect Immunofluorescence Test for Antinuclear Antibodies.


Journal

Annals of clinical and laboratory science
ISSN: 1550-8080
Titre abrégé: Ann Clin Lab Sci
Pays: United States
ID NLM: 0410247

Informations de publication

Date de publication:
Mar 2020
Historique:
entrez: 6 5 2020
pubmed: 6 5 2020
medline: 4 2 2021
Statut: ppublish

Résumé

The performance of the automated indirect immunofluorescence system was compared with the manual method for detection of antinuclear antibodies (ANA) from 354 clinical serum samples. We compared the results (negative or positive), ANA patterns, and titers for the two methods. The coincidence rates for ANA positive and negative samples were 93.4% and 98.7%, respectively. The coincidence rates for single patterns, mixed patterns, and the final titers were 85.1%, 87.6%, and 87.6%, respectively. The homogeneous, speckled, cytoplasmic, centromere, multiple nuclear dots, and nucleolar pattern coincidence rates were 79.3%, 83.0%, 87.8%, 72.7%, 50%, and 56.3%, respectively. The automated indirect immunofluorescence system had acceptable accuracy for detection of ANA.

Identifiants

pubmed: 32366571
pii: 50/2/282

Substances chimiques

Antibodies, Antinuclear 0
Autoantibodies 0

Types de publication

Evaluation Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

282-286

Informations de copyright

© 2020 by the Association of Clinical Scientists, Inc.

Auteurs

Shiji Wu (S)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China wilson547@163.com.

Feng Liu (F)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Feng Wang (F)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Jin Huang (J)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Botao Yin (B)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Min Huang (M)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Ting Wang (T)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Na Wei (N)

Department of Clinical Laboratory, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

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