SARS-CoV-2 detection in formalin-fixed paraffin-embedded tissue specimens from surgical resection of tongue squamous cell carcinoma.
Betacoronavirus
/ genetics
COVID-19
COVID-19 Testing
Carcinoma, Squamous Cell
/ complications
Clinical Laboratory Techniques
/ methods
Coronavirus Infections
/ diagnosis
Fixatives
Formaldehyde
Humans
Male
Middle Aged
Pandemics
Paraffin Embedding
Pneumonia, Viral
/ diagnosis
Postoperative Complications
/ diagnosis
RNA, Viral
/ analysis
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
Tissue Fixation
/ methods
Tissue Preservation
/ methods
Tongue Neoplasms
/ complications
pathology, molecular
pathology, oral
viruses
Journal
Journal of clinical pathology
ISSN: 1472-4146
Titre abrégé: J Clin Pathol
Pays: England
ID NLM: 0376601
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
15
04
2020
revised:
17
04
2020
accepted:
17
04
2020
pubmed:
6
5
2020
medline:
30
10
2020
entrez:
6
5
2020
Statut:
ppublish
Résumé
In the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, pathologists can be exposed to infection handling surgical specimens. Guidelines related to safety procedures in the laboratory have been released. However, there is a lack of studies performed on biopsy and surgical resection specimens. Here we report the detection of SARS-CoV-2 in formalin-fixed paraffin-embedded samples from surgical resection of tongue squamous cell carcinoma of a patient who developed COVID-19 postsurgery. RNA of SARS-CoV-2 strain was detected in the tumour and the normal submandibular gland samples using real-time PCR-based assay. No viral RNA was found in metastatic and reactive lymph nodes. We demonstrated that SARS-CoV-2 RNA can be detected in routine histopathological samples even before COVID-19 disease development. These findings may give important information on the possible sites of infection or virus reservoir, and highlight the necessity of proper handling and fixation before sample processing.
Identifiants
pubmed: 32366599
pii: jclinpath-2020-206635
doi: 10.1136/jclinpath-2020-206635
doi:
Substances chimiques
Fixatives
0
RNA, Viral
0
Formaldehyde
1HG84L3525
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
754-757Informations de copyright
© Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.