Effects of Schizochytrium and micro-minerals on immune, antioxidant, inflammatory and lipid-metabolism status of Micropterus salmoides fed high- and low-fishmeal diets.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 05 2020
04 05 2020
Historique:
received:
28
06
2019
accepted:
09
04
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
7
1
2021
Statut:
epublish
Résumé
A 12-week factorial experiment was conducted to investigate the interactive effects of dietary algal meal (Schizochytrium sp., AM) and micro-minerals (MM, either organic [OM] or inorganic [IM]) on the immune and antioxidant status, and the expression of hepatic genes involved in the regulation of antioxidants, inflammatory cytokines, lipid metabolism, and organ growth of largemouth bass (LMB; Micropterus salmoides) fed high-and low-fishmeal (FM) diets. For this purpose, two sets of six iso-nitrogenous (42% crude protein) and iso-lipidic (12% lipid) diets, such as high (35%) and low (10%) FM diets were formulated. Within each FM level, AM was used to replace 50% or 100% of fish oil (FO), or without AM (FO control) and supplemented with either OM or IM (Fe, Zn, Mn, Cu, and Se). Diets were fed to juvenile LMB (initial weight, 25.87 ± 0.08 g) to near satiation twice daily. The results indicated that FO replacement by dietary AM did not change the levels of most biochemical (ALB, AMY, TP and GLOB), antioxidants (SOD, GPx and GSH), and immune (IgM and lysozyme) parameters in LMB, except ALP and CAT. MM affected only hepatic GSH, with lower values in fish fed the OM diets. FM influenced the levels of ALP, AMY, GLOB, IgM, and MDA (P < 0.05). A three-way interactive effect (P = 0.016) was found on IgM only, with lower levels in fish fed diet 12 (low-FM, AM100, OM). Subsequently, the relative expressions of hepatic antioxidants (Cu/Zn-SOD and GPx-4), inflammatory cytokines (TNF-α and TGF-β1), lipid metabolism (FASN and CYP7A1), and organ growth (IGF-I) related genes were affected by the dietary treatments, with interactions being present in Cu/Zn-SOD, TNF-α, TGF-β1, FASN and IGF-I. Overall, dietary AM could be used as an alternative to FO in low-FM diets without compromising the health of LMB, especially when it is supplemented with MM.
Identifiants
pubmed: 32366883
doi: 10.1038/s41598-020-64286-9
pii: 10.1038/s41598-020-64286-9
pmc: PMC7198547
doi:
Substances chimiques
Antioxidants
0
Cytokines
0
Fish Oils
0
Fish Proteins
0
Minerals
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7457Références
Miller, M. R., Nichols, P. D. & Carter, C. G. Replacement of fish oil with thraustochytrid Schizochytrium sp. L oil in Atlantic salmon parr (Salmo salar L) diets. Comparative biochemistry and physiology part A: Molecular & integrative physiology. 148, 382–392 (2007).
doi: 10.1016/j.cbpa.2007.05.018
Jones, A. C. et al. Prioritization of knowledge needs for sustainable aquaculture: a national and global perspective. Fish and fisheries. 16, 668–683 (2015).
doi: 10.1111/faf.12086
Heal, G. & Schlenker, W. Economics: sustainable fisheries. Nature. 455, 1044–1045 (2008).
doi: 10.1038/4551044a
Kumar, V. et al. Replacement of fish oil with Schizochytrium meal and its impacts on the growth and lipid metabolism of Pacific white shrimp (Litopenaeus vannamei). Aquaculture nutrition. 24, 1769–1781 (2018).
doi: 10.1111/anu.12816
Fontagné-Dicharry, S. et al. Influence of the forms and levels of dietary selenium on antioxidant status and oxidative stress-related parameters in rainbow trout (Oncorhynchus mykiss) fry. British journal of nutrition. 113, 1876–1887 (2015).
pubmed: 25990817
doi: 10.1017/S0007114515001300
Prabhu, P. A. J. et al. Responses in micro-mineral metabolism in rainbow trout to change in dietary ingredient composition and inclusion of a micro-mineral premix. PloS one. 11, e0149378 (2016).
doi: 10.1371/journal.pone.0149378
Kousoulaki, K., Mørkøre, T., Nengas, I., Berge, R. K. & Sweetman, J. Microalgae and organic minerals enhance lipid retention efficiency and fillet quality in Atlantic salmon (Salmo salar L.). Aquaculture. 451, 47–57 (2016).
doi: 10.1016/j.aquaculture.2015.08.027
Habte-Tsion, H.-M. et al. A deficiency or an excess of dietary threonine level affects weight gain, enzyme activity, immune response and immune-related gene expression in juvenile blunt snout bream (Megalobrama amblycephala). Fish & shellfish immunology. 42, 439–446 (2015).
doi: 10.1016/j.fsi.2014.11.021
Habte-Tsion, H.-M. et al. Threonine modulates immune response, antioxidant status and gene expressions of antioxidant enzymes and antioxidant-immune-cytokine-related signaling molecules in juvenile blunt snout bream (Megalobrama amblycephala). Fish & shellfish immunology. 51, 189–199 (2016).
doi: 10.1016/j.fsi.2015.11.033
Hulefeld, R. et al. Nutritional evaluation of an improved soybean meal as a fishmeal replacer in the diet of Pacific white shrimp, Litopenaeus vannamei. Aquaculture research. 49, 1414–1422 (2018).
doi: 10.1111/are.13593
Bayir, A. et al. Metabolic responses to prolonged starvation, food restriction, and refeeding in the brown trout, Salmo trutta: oxidative stress and antioxidant defenses. Comparative biochemistry and physiology part B: Biochemistry and molecular biology. 159, 191–196 (2011).
doi: 10.1016/j.cbpb.2011.04.008
Abele, D. & Puntarulo, S. Formation of reactive species and induction of antioxidant defence systems in polar and temperate marine invertebrates and fish. Comparative biochemistry and physiology part A: Molecular & integrative physiology. 138, 405–415 (2004).
doi: 10.1016/j.cbpb.2004.05.013
Martínez-Álvarez, R. M., Morales, A. E. & Sanz, A. Antioxidant defenses in fish: biotic and abiotic factors. Reviews in fish biology and fisheries. 15, 75–88 (2005).
doi: 10.1007/s11160-005-7846-4
Wilhelm Filho, D. et al. Seasonal changes in antioxidant defenses of the digestive gland of the brown mussel (Perna perna). Aquaculture. 203, 149–158 (2001).
doi: 10.1016/S0044-8486(01)00599-3
Furukawa, S. et al. Increased oxidative stress in obesity and its impact on metabolic syndrome. The journal of clinical investigation. 114, 1752–1761 (2017).
doi: 10.1172/JCI21625
Picha, M. E., Turano, M. J., Beckman, B. R. & Borski, R. J. Endocrine biomarkers of growth and applications to aquaculture: A minireview of growth hormone, insulin-like growth factor (IGF)-I, and IGF-binding proteins as potential growth indicators in fish. North american journal of aquaculture. 70, 196–211 (2008).
doi: 10.1577/A07-038.1
Tidwell, J. H., Webster, C. D. & Coyle, S. D. Effects of dietary protein level on second year growth and water quality for largemouth bass (Micropterus salmoides) raised in ponds. Aquaculture. 145, 213–223 (1996).
doi: 10.1016/S0044-8486(96)01356-7
Chen, Y.-J. et al. Dietary vitamin C requirement and its effects on tissue antioxidant capacity of juvenile largemouth bass, Micropterus salmoides. Aquaculture. 435, 431–436 (2015).
doi: 10.1016/j.aquaculture.2014.10.013
Xie, Y.-R. et al. Effect of dietary vitamin C on growth and non-specific immunity in largemouth bass Micropterus salmoides. Journal of dalian fisheries university. 22, 249–254 (2007).
Zhou, H., Chen, N., Qiu, X., Zhao, M. & Jin, L. Arginine requirement and effect of arginine intake on immunity in largemouth bass, Micropterus salmoides. Aquaculture nutrition. 18, 107–116 (2012).
doi: 10.1111/j.1365-2095.2011.00886.x
Zhu, Y. et al. Effect of dietary selenium level on growth performance, body composition and hepatic glutathione peroxidase activities of largemouth bass Micropterus salmoide. Aquaculture research. 43, 1660–1668 (2012).
doi: 10.1111/j.1365-2109.2011.02972.x
Zhu, T. et al. Effects of dietary lipid level on morphology indexes, tissue fatty acid composition, serum biochemical indexes and liver antioxidant indexes of largemouth bass (Micropterus salmoides). Chinese journal of animal nutrition. 30, 126–137 (2018).
Chen, N., Jin, L., Zhou, H. Y. & Qiu, X. J. Effects of dietary arginine levels and carbohydrate-to-lipid ratios on mRNA expression of growth-related hormones in largemouth bass, Micropterus salmoides. General and comparative endocrinology. 179, 121–127 (2012).
pubmed: 22906421
doi: 10.1016/j.ygcen.2012.08.004
Chen, Y. J. et al. Effect of dietary oxidized fish oil on growth performance, body composition, antioxidant defence mechanism and liver histology of juvenile largemouth bass Micropterus salmoides. Aquaculture nutrition. 18, 321–331 (2012).
doi: 10.1111/j.1365-2095.2011.00900.x
Chen, Y. J. et al. Effect of dietary vitamin E and selenium supplementation on growth, body composition, and antioxidant defense mechanism in juvenile largemouth bass (Micropterus salmoide) fed oxidized fish oil. Fish physiology and biochemistry. 39, 593–604 (2013).
pubmed: 23053606
doi: 10.1007/s10695-012-9722-1
Subhadra, B., Lochmann, R., Rawles, S. & Chen, R. Effect of dietary lipid source on the growth, tissue composition and hematological parameters of largemouth bass (Micropterus salmoides). Aquaculture. 255, 210–222 (2006).
doi: 10.1016/j.aquaculture.2005.11.043
Li, S., Lian, X., Chen, N., Wang, M. & Sang, S. Effects of dietary vitamin E level on growth performance, feed utilization, antioxidant capacity and nonspecific immunity of largemouth bass, Micropterus salmoides. Aquaculture nutrition. 24, 1679–1688 (2018).
doi: 10.1111/anu.12802
Yu, L. et al. Dietary butylated hydroxytoluene improves lipid metabolism, antioxidant and anti-apoptotic response of largemouth bass (Micropterus salmoides). Fish & shellfish immunology. 72, 220–229 (2018).
doi: 10.1016/j.fsi.2017.10.054
Yu, H. et al. Dietary supplementation of Grobiotic®-A increases short-term inflammatory responses and improves long-term growth performance and liver health in largemouth bass (Micropterus salmoides). Aquaculture. 500, 327–337 (2019).
doi: 10.1016/j.aquaculture.2018.10.033
Food and Agriculture Organization of the United Nations (FAO). The State of World Fisheries and Aquaculture, FAO, Italy, Rome (2004).
Smolelis, A. & Hartsell, S. The determination of lysozyme. Journal of bacteriology. 58, 731 (1949).
pubmed: 15395173
pmcid: 385699
doi: 10.1128/JB.58.6.731-736.1949
Sun, Y., Yang, H., Ma, R. & Lin, W. Probiotic applications of two dominant gut Bacillus strains with antagonistic activity improved the growth performance and immune responses of grouper Epinephelus coioides. Fish & shellfish immunology. 29, 803–809 (2010).
doi: 10.1016/j.fsi.2010.07.018
Habte-Tsion, H.-M. et al. Threonine affects digestion capacity and hepatopancreatic gene expression of juvenile blunt snout bream (Megalobrama amblycephala). British journal of nutrition. 114, 533–543 (2015).
pubmed: 26202077
doi: 10.1017/S0007114515002196
Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2
pubmed: 11846609
doi: 10.1006/meth.2001.1262
Kader, M. A., Koshio, S., Ishikawa, M., Yokoyama, S. & Bulbul, M. Supplemental effects of some crude ingredients in improving nutritive values of low fishmeal diets for red sea bream, Pagrus major. Aquaculture. 308, 136–144 (2010).
doi: 10.1016/j.aquaculture.2010.07.037
Sandnes, K., Lie, Ø. & Waagbø, R. Normal ranges of some blood chemistry parameters in adult farmed Atlantic salmon, Salmo salar. Journal of fish biology. 32, 129–136 (1988).
doi: 10.1111/j.1095-8649.1988.tb05341.x
Sharma, U., Pal, D. & Prasad, R. Alkaline phosphatase: an overview. Indian journal of clinical biochemistry. 29, 269–278 (2014).
pubmed: 24966474
doi: 10.1007/s12291-013-0408-y
Severini, G., Aliberti, L. M. & Di Giovannandrea, R. Diagnostic aspects of alkaline phosphatase: separation of isoenzymes in normal and pathological human serum by high-performance liquid chromatography. Journal of chromatography B: Biomedical sciences and applications. 563, 147–152 (1991).
doi: 10.1016/0378-4347(91)80287-M
Savova, M. & Kirev, T. Alkaline phosphatase activity in serum of guinea fowl bearing bone tumours induced by osteopetrosis virus strain PTS-56. Avian pathology. 21, 667–673 (1992).
pubmed: 18670985
doi: 10.1080/03079459208418888
pmcid: 18670985
Bag, M. R., Makesh, M., Rajendran, K. V. & Mukherjee, S. C. Characterization of IgM of Indian major carps and their cross-reactivity with anti-fish IgM antibodies. Fish & shellfish immunology. 26, 275–278 (2009).
doi: 10.1016/j.fsi.2008.11.009
Uribe, C., Folch, H., Enriquez, R. & Moran, G. Innate and adaptive immunity in teleost fish: a review. Veterinarni medicina. 56, 486–503 (2011).
doi: 10.17221/3294-VETMED
Kousoulaki, K. et al. Metabolism, health and fillet nutritional quality in Atlantic salmon (Salmo salar) fed diets containing n-3-rich microalgae. Journal of nutritional science. 4, 1–13 (2015).
doi: 10.1017/jns.2015.14
Gaweł, S., Wardas, M., Niedworok, E. & Wardas, P. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci lekarskie (Warsaw, Poland: 1960). 57, 453–455 (2004).
Nogueira, C. W., Quinhones, E. B., Jung, E. A. C., Zeni, G. & Rocha, J. B. T. Anti-inflammatory and antinociceptive activity of diphenyl diselenide. Inflammation research. 52, 56–63 (2003).
pubmed: 12665122
doi: 10.1007/s000110300001
Wang, B. et al. Effects of dietary arginine supplementation on growth performance, flesh quality, muscle antioxidant capacity and antioxidant-related signalling molecule expression in young grass carp (Ctenopharyngodon idella). Food chemistry. 167, 91–99 (2015).
pubmed: 25148964
doi: 10.1016/j.foodchem.2014.06.091
Fontagné-Dicharry, S. et al. Antioxidant defense system is altered by dietary oxidized lipid in first-feeding rainbow trout (Oncorhynchus mykiss). Aquaculture. 424, 220–227 (2014).
doi: 10.1016/j.aquaculture.2014.01.009
Domínguez, D. et al. Inorganic, organic, and encapsulated minerals in vegetable meal based diets for Sparus aurata (Linnaeus, 1758). PeerJ. 5, e3710, https://doi.org/10.7717/peerj.3710 (2017).
doi: 10.7717/peerj.3710
pubmed: 29093992
pmcid: 5661455
Benson, J. M. & Shepherd, D. M. Dietary ligands of the aryl hydrocarbon receptor induce anti-inflammatory and immunoregulatory effects on murine dendritic cells. Toxicological sciences. 124, 327–338 (2011).
pubmed: 21948866
pmcid: 3216417
doi: 10.1093/toxsci/kfr249
Rebl, A., Goldammer, T. & Seyfert, H.-M. Toll-like receptor signaling in bony fish. Veterinary immunology and immunopathology. 134, 139–150 (2010).
pubmed: 19850357
doi: 10.1016/j.vetimm.2009.09.021
Menendez, J. A. & Lupu, R. Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis. Nature reviews cancer. 7, 763–777 (2007).
pubmed: 17882277
doi: 10.1038/nrc2222
Out, C. et al. Liver receptor homolog-1 is critical for adequate up-regulation of Cyp7a1 gene transcription and bile salt synthesis during bile salt sequestration. Hepatology. 53, 2075–2085 (2011).
pubmed: 21391220
doi: 10.1002/hep.24286
Russell, D. W. & Setchell, K. D. Bile acid biosynthesis. Biochemistry. 31, 4737–4749 (1992).
pubmed: 1591235
doi: 10.1021/bi00135a001
Kim, I. et al. Differential regulation of bile acid homeostasis by the farnesoid X receptor in liver and intestine. Journal of lipid research. 48, 2664–2672 (2007).
pubmed: 17720959
doi: 10.1194/jlr.M700330-JLR200
Zhou, Q. L. et al. Graded replacing fishmeal with canola meal in diets affects growth and target of rapamycin pathway gene expression of juvenile blunt snout bream, Megalobrama amblycephala. Aquaculture nutrition. 24, 300–309 (2018).
doi: 10.1111/anu.12560
Wilson-Arop, O. M. et al. Dietary histidine requirement of juvenile blunt snout bream (Megalobrama amblycephala). Aquaculture nutrition. 24, 1122–1132 (2018).
doi: 10.1111/anu.12651
Moon, H. Y. & Gatlin, D. M. III Total sulfur amino acids requirement of juvenile red drum, Sciaenops ocellatus. Aquaculture. 95, 97–106 (1991).
doi: 10.1016/0044-8486(91)90076-J