Inflammescent CX3CR1+CD57+CD8+ T cells are generated and expanded by IL-15.


Journal

JCI insight
ISSN: 2379-3708
Titre abrégé: JCI Insight
Pays: United States
ID NLM: 101676073

Informations de publication

Date de publication:
04 06 2020
Historique:
received: 23 08 2019
accepted: 30 04 2020
pubmed: 6 5 2020
medline: 19 5 2021
entrez: 6 5 2020
Statut: epublish

Résumé

HIV infection is associated with an increase in the proportion of activated CD8+ memory T cells (Tmem) that express CX3CR1, but how these cells are generated and maintained in vivo is unclear. We demonstrate that increased CX3CR1 expression on CD8+ Tmem in people living with HIV (PLWH) is dependent on coinfection with human CMV, and CX3CR1+CD8+ Tmem are enriched for a putatively immunosenescent CD57+CD28- phenotype. The cytokine IL-15 promotes the phenotype, survival, and proliferation of CX3CR1+CD57+CD8+ Tmem in vitro, whereas T cell receptor stimulation leads to their death. IL-15-driven survival is dependent on STAT5 and Bcl-2 activity, and IL-15-induced proliferation requires STAT5 and mTORC1. Thus, we identify mechanistic pathways that could explain how "inflammescent" CX3CR1+CD57+ CD8+ Tmem dominate the overall memory T cell pool in CMV-seropositive PLWH and that support reevaluation of immune senescence as a nonproliferative dead end.

Identifiants

pubmed: 32369455
pii: 132963
doi: 10.1172/jci.insight.132963
pmc: PMC7346586
doi:
pii:

Substances chimiques

CD57 Antigens 0
CX3C Chemokine Receptor 1 0
CX3CR1 protein, human 0
IL15 protein, human 0
Interleukin-15 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : UM1 AI069501
Pays : United States
Organisme : CSRD VA
ID : IK2 CX001471
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI076174
Pays : United States
Organisme : NICHD NIH HHS
ID : R21 HD094646
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI069501
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL134544
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI036214
Pays : United States
Organisme : NIAID NIH HHS
ID : R24 AI106039
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI036219
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI131385
Pays : United States

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Auteurs

Stephen R Morris (SR)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.
Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA.

Bonnie Chen (B)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

Joseph C Mudd (JC)

Barrier Immunity Section, Laboratory of Viral Diseases, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA.

Soumya Panigrahi (S)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

Carey L Shive (CL)

Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA.

Scott F Sieg (SF)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

Cheryl M Cameron (CM)

Center for AIDS Research, Department of Nutrition, Case Western Reserve University, Cleveland, Ohio, USA.

David A Zidar (DA)

Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA.

Nicholas T Funderburg (NT)

Division of Medical Laboratory Science, School of Health and Rehabilitation Sciences, Ohio State University, Columbus, Ohio, USA.

Souheil-Antoine Younes (SA)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

Benigno Rodriguez (B)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

Sara Gianella (S)

Center for AIDS Research, Division of Infectious Diseases and Global Public Health, Department of Medicine, University of California, San Diego, La Jolla, California, USA.

Michael M Lederman (MM)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

Michael L Freeman (ML)

Center for AIDS Research, Division of Infectious Diseases and HIV Medicine, Department of Medicine, Case Western Reserve University/University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA.

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Classifications MeSH