The Robust Restriction of Zika Virus by Type-I Interferon in A549 Cells Varies by Viral Lineage and Is Not Determined by IFITM3.
IFITM3
Zika virus
interferon-stimulated genes
type-I interferon
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
02 05 2020
02 05 2020
Historique:
received:
09
04
2020
revised:
29
04
2020
accepted:
30
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
23
2
2021
Statut:
epublish
Résumé
Type-I interferon (IFN-I) is a major antiviral host response but its impact on Zika virus (ZIKV) replication is not well defined, particularly as it relates to different circulating strains. Interferon stimulated genes (ISGs) that inhibit ZIKV, such as IFITM3, have been identified largely using overexpression studies. Here, we tested whether diverse ZIKV strains differed in their susceptibility to IFN-I-mediated restriction and the contribution of IFITM3 to this restriction. We identified a robust IFN-I-mediated antiviral effect on ZIKV replication (>100-fold reduction) in A549 cells, a commonly used cell line to study ZIKV replication. The extent of inhibition depended on the IFN-I type and the virus strain tested. Viruses from the American pathogenic outbreak were more sensitive to IFNα (
Identifiants
pubmed: 32370187
pii: v12050503
doi: 10.3390/v12050503
pmc: PMC7290589
pii:
doi:
Substances chimiques
IFITM3 protein, human
0
Interferon Type I
0
Membrane Proteins
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Institute of Allergy and Infectious Diseases
ID : K99AI125136
Pays : International
Organisme : National Institute of Allergy and Infectious Diseases
ID : T32AI083203
Pays : International
Organisme : National Institute of Allergy and Infectious Diseases
ID : R01AI076105
Pays : International
Organisme : National Institute of Allergy and Infectious Diseases
ID : F30AI142870
Pays : International
Organisme : NIAID NIH HHS
ID : F30 AI142870
Pays : United States
Déclaration de conflit d'intérêts
The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Références
J Infect Dis. 2017 Dec 16;216(suppl_10):S906-S911
pubmed: 29267924
Nat Immunol. 2013 Sep;14(9):901-7
pubmed: 23872679
Nat Microbiol. 2018 Nov;3(11):1214-1223
pubmed: 30224801
PLoS Pathog. 2017 Mar 9;13(3):e1006219
pubmed: 28278237
Cell Host Microbe. 2016 May 11;19(5):720-30
pubmed: 27066744
Annu Rev Virol. 2014 Nov 1;1:261-283
pubmed: 25599080
Nat Protoc. 2017 Jun;12(6):1261-1276
pubmed: 28538739
Proc Natl Acad Sci U S A. 2017 Jan 24;114(4):E590-E599
pubmed: 28069935
Sci Signal. 2018 Jun 19;11(535):
pubmed: 29921658
N Engl J Med. 2016 Mar 10;374(10):951-8
pubmed: 26862926
Clin Microbiol Infect. 2014 Oct;20(10):O595-6
pubmed: 24909208
Nat Commun. 2018 Jan 29;9(1):414
pubmed: 29379028
Cell. 2017 Dec 14;171(7):1573-1588.e28
pubmed: 29224777
PLoS Negl Trop Dis. 2016 Feb 25;10(2):e0004517
pubmed: 26914330
Trends Immunol. 2015 Mar;36(3):139-49
pubmed: 25687684
Cell Rep. 2016 Jun 14;15(11):2323-30
pubmed: 27268505
N Engl J Med. 2009 Jun 11;360(24):2536-43
pubmed: 19516034
J Infect Dis. 2017 Dec 16;216(suppl_10):S897-S905
pubmed: 29267923
Science. 2014 Jan 3;343(6166):84-87
pubmed: 24336571
Infect Genet Evol. 2017 Apr;49:134-137
pubmed: 28095299
MMWR Morb Mortal Wkly Rep. 2016 Jan 29;65(3):59-62
pubmed: 26820244
J Vis Exp. 2016 Aug 23;(114):
pubmed: 27584546
Nature. 2018 Jun;558(7711):610-614
pubmed: 29925952
PLoS Negl Trop Dis. 2017 Jul 3;11(7):e0005704
pubmed: 28672028
J Virol. 2015 Sep;89(17):8880-96
pubmed: 26085147
Stem Cell Reports. 2017 Mar 14;8(3):715-727
pubmed: 28216147
J Mol Biol. 2007 Feb 16;366(2):525-39
pubmed: 17174979
Cell Stem Cell. 2017 Mar 2;20(3):397-406.e5
pubmed: 28132835
J Infect Dis. 2017 Dec 16;216(suppl_10):S891-S896
pubmed: 29267916
Cell Stem Cell. 2016 May 5;18(5):587-90
pubmed: 26952870
Front Immunol. 2019 Aug 16;10:1928
pubmed: 31474994
Cell. 2017 May 4;169(4):610-620.e14
pubmed: 28457610
Virology. 2016 Jun;493:217-26
pubmed: 27060565
Cell Host Microbe. 2018 May 9;23(5):672-685.e6
pubmed: 29746837
PLoS Pathog. 2017 Mar 9;13(3):e1006258
pubmed: 28278235
PLoS Pathog. 2017 Feb 2;13(2):e1006164
pubmed: 28152048
Clin Microbiol Rev. 2016 Jul;29(3):487-524
pubmed: 27029595
Cell Stem Cell. 2008 Jan 10;2(1):90-102
pubmed: 18371425
Annu Rev Virol. 2019 Sep 29;6(1):567-584
pubmed: 31283436
Nat Rev Immunol. 2015 Feb;15(2):87-103
pubmed: 25614319
Cell. 2009 Dec 24;139(7):1243-54
pubmed: 20064371
Nat Rev Immunol. 2014 Jan;14(1):36-49
pubmed: 24362405
Nucleic Acids Res. 2016 Oct 14;44(18):8610-8620
pubmed: 27580721
Br J Pharmacol. 2013 Mar;168(5):1048-58
pubmed: 23072338
Proc Natl Acad Sci U S A. 2017 Feb 28;114(9):E1587-E1596
pubmed: 28193876
mSphere. 2017 Jul 26;2(4):
pubmed: 28815211
Virology. 2017 Dec;512:180-186
pubmed: 28972927
PLoS Negl Trop Dis. 2017 Sep 21;11(9):e0005821
pubmed: 28934211
EMBO J. 2017 Jun 14;36(12):1653-1668
pubmed: 28473450
Sci Rep. 2017 Jun 30;7(1):4475
pubmed: 28667332
PLoS One. 2018 Jul 9;13(7):e0200086
pubmed: 29985932
Emerg Microbes Infect. 2016 Aug 24;5:e93
pubmed: 27553173
Cell Host Microbe. 2016 May 11;19(5):705-12
pubmed: 27066743
J Virol. 2019 Jun 14;93(13):
pubmed: 31019057
Cytokine. 2011 Nov;56(2):282-9
pubmed: 21856167
Syst Biol. 2010 May;59(3):307-21
pubmed: 20525638
EBioMedicine. 2016 Oct;12:161-169
pubmed: 27688094
Nat Chem Biol. 2019 Mar;15(3):259-268
pubmed: 30643282
Annu Rev Immunol. 2014;32:513-45
pubmed: 24555472
Circ Res. 2016 Nov 11;119(11):1183-1189
pubmed: 27650556