Evaluating the circadian rhythm and response to glucose addition in dispersed growth cultures of Neurospora crassa.
Circadian clock
Glucose utilization
Luciferase reporter
Polyacrylic acid
Journal
Fungal biology
ISSN: 1878-6146
Titre abrégé: Fungal Biol
Pays: Netherlands
ID NLM: 101524465
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
06
09
2019
revised:
12
11
2019
accepted:
13
11
2019
entrez:
12
5
2020
pubmed:
12
5
2020
medline:
21
8
2021
Statut:
ppublish
Résumé
Work on the filamentous fungus Neurospora crassa has contributed to or pioneered many aspects of research on circadian clock mechanism, a process that is functionally conserved across eukaryotes. Biochemical assays of the fungal circadian clock typically involve growth in liquid medium where Neurospora forms a spherical ball of submerged mycelium. Here, we revive a method for dispersed growth of Neurospora in batch culture using polyacrylic acid as an additive to the medium. We demonstrate that dispersed growth cultures utilize more carbon than mycelial balls, but nonetheless retain a functional circadian clock. This culturing method is suited for use in circadian experiments where uniform exposure to nutrients and/or increased biomass is required.
Identifiants
pubmed: 32389302
pii: S1878-6146(19)30162-X
doi: 10.1016/j.funbio.2019.11.004
pmc: PMC7217977
mid: NIHMS1545390
pii:
doi:
Substances chimiques
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
398-406Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM128252
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118021
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118022
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB022546
Pays : United States
Informations de copyright
Copyright © 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflict of interest.
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