[Doxycycline Sensitive Two-Promoter Integrator Based on the TET-ON 3G Transactivator].


Journal

Molekuliarnaia biologiia
ISSN: 0026-8984
Titre abrégé: Mol Biol (Mosk)
Pays: Russia (Federation)
ID NLM: 0105454

Informations de publication

Date de publication:
Historique:
received: 10 10 2019
accepted: 05 11 2019
entrez: 12 5 2020
pubmed: 12 5 2020
medline: 20 8 2020
Statut: ppublish

Résumé

A synthetic genetic chain was created-a two-promoter integrator, the activity of the output signal of which is high only with the high activity of two promoters at the input of the chain. The integrator is shown to work by the example of cancer-specific promoters of the human survivin and telomerase genes that can be used for specific transcription of target genes in tumor cells. To create the integrator, we used the well-known TET-ON 3G transactivator, regulated by doxycycline.

Identifiants

pubmed: 32392201
doi: 10.31857/S0026898420020056
doi:

Substances chimiques

BIRC5 protein, human 0
Survivin 0
Trans-Activators 0
Telomerase EC 2.7.7.49
Tetracycline F8VB5M810T
Doxycycline N12000U13O

Types de publication

Journal Article

Langues

rus

Sous-ensembles de citation

IM

Pagination

308-312

Auteurs

Yu M Khodarovich (YM)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russia.
khodarovich@mail.ru.

D D Rakhmaninova (DD)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russia.

A M Barishnikova (AM)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russia.

S M Deyev (SM)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, 117997 Russia.

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Classifications MeSH