The circular RNA circMAST1 promotes hepatocellular carcinoma cell proliferation and migration by sponging miR-1299 and regulating CTNND1 expression.

Animals Carcinoma, Hepatocellular / genetics Catenins / genetics Cell Movement Cell Proliferation Databases, Genetic Female Gene Expression Regulation, Neoplastic Hep G2 Cells Humans Liver Neoplasms / genetics Male Mice, Inbred BALB C Mice, Nude MicroRNAs / genetics Middle Aged Neoplasm Invasiveness RNA, Circular / genetics Signal Transduction Tumor Burden Delta Catenin

Journal

Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092

Informations de publication

Date de publication:
11 05 2020
Historique:
received: 15 01 2020
accepted: 14 04 2020
revised: 11 04 2020
entrez: 13 5 2020
pubmed: 13 5 2020
medline: 10 3 2021
Statut: epublish

Résumé

Circular RNAs (circRNAs) are a class of non-coding RNAs with a loop structure; however, their functions remain largely unknown. Growing evidence suggests that circRNAs play a pivotal role in the progression of malignant diseases. However, the expression profiles and function of circRNAs in hepatocellular carcinoma (HCC) remain unclear. We investigated the expression of microtubule-associated serine/threonine kinase 1 (MAST1) circRNA (circMAST1) in HCC and healthy tissues using bioinformatics, quantitative real-time PCR (qRT-PCR), and fluorescence in situ hybridization. Luciferase reporter assays were performed to assess the interaction between circMAST1 and miR-1299. Proliferation assays, colony formation assays, flow cytometry, transwell assays, and western blotting were also performed. A mouse xenograft model was also used to determine the effect of circMAST1 on HCC growth in vivo. CircMAST1 was upregulated in HCC tissues and cell lines; silencing via small interfering RNA inhibited migration, invasion, and proliferation of HCC cell lines in vitro as well as tumor growth in vivo. Furthermore, the expression of circMAST1 was positively correlated with catenin delta-1 (CTNND1) and negatively correlated with microRNA (miR)-1299 in HCC clinical samples. Importantly, circMAST1 sponged miR-1299 to stabilize the expression of CTNND1 and promoted tumorigenic features in HCC cell lines. We found that circMAST1 may serve as a novel biomarker for HCC. Moreover, circMAST1 elicits HCC progression by sponging miRNA-1299 and stabilizing CTNND1. Our data provide potential options for therapeutic targets in patients with HCC.

Identifiants

DOI: 10.1038/s41419-020-2532-y PMID: 32393764 PMC: PMC7214424
pubmed: 32393764
doi: 10.1038/s41419-020-2532-y
pii: 10.1038/s41419-020-2532-y
pmc: PMC7214424
doi:

Substances chimiques

Catenins 0
MIRN1299 microRNA, human 0
MicroRNAs 0
RNA, Circular 0
Delta Catenin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

340

Références

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Auteurs

Xiufeng Yu (X)

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, 150081, China.
College of Medical Laboratory Science and Technology, Harbin Medical University (Daqing), Daqing, 163319, China.
Central Laboratory of Harbin Medical University (Daqing), Daqing, 163319, China.

Ping Sheng (P)

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, 150081, China.

Jing Sun (J)

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, 150081, China.

Xijuang Zhao (X)

College of Medical Laboratory Science and Technology, Harbin Medical University (Daqing), Daqing, 163319, China.
Central Laboratory of Harbin Medical University (Daqing), Daqing, 163319, China.

Junting Zhang (J)

College of Medical Laboratory Science and Technology, Harbin Medical University (Daqing), Daqing, 163319, China.
Central Laboratory of Harbin Medical University (Daqing), Daqing, 163319, China.

Yiying Li (Y)

College of Medical Laboratory Science and Technology, Harbin Medical University (Daqing), Daqing, 163319, China.
Central Laboratory of Harbin Medical University (Daqing), Daqing, 163319, China.

YiMeng Zhang (Y)

College of Bioinformatics and Technology, Harbin Medical University (Daqing), Daqing, 163319, China.

Wenxiu Zhang (W)

College of Medical Laboratory Science and Technology, Harbin Medical University (Daqing), Daqing, 163319, China.

Jianqi Wang (J)

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, 150081, China.

Kunpeng Liu (K)

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, 150081, China.

Daling Zhu (D)

College of Medical Laboratory Science and Technology, Harbin Medical University (Daqing), Daqing, 163319, China. dalingz@yahoo.com.
Central Laboratory of Harbin Medical University (Daqing), Daqing, 163319, China. dalingz@yahoo.com.

Hongchi Jiang (H)

Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Key Laboratory of Hepatosplenic Surgery, Ministry of Education, Harbin, 150081, China. jianghongchi@hrbmu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › The circular RNA circMAST1 promotes...
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies du foie › Tumeurs du foie › Carcinome hépatocellulaire › The circular RNA circMAST1 promotes...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Caténines › The circular RNA circMAST1 promotes...
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questionsmedicales.fr › Maladies de l'appareil digestif › Maladies du foie › Tumeurs du foie › The circular RNA circMAST1 promotes...
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