Structure of a cyanobacterial photosystem I surrounded by octadecameric IsiA antenna proteins.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
11 05 2020
11 05 2020
Historique:
received:
27
09
2019
accepted:
17
04
2020
entrez:
13
5
2020
pubmed:
13
5
2020
medline:
16
6
2021
Statut:
epublish
Résumé
Iron-stress induced protein A (IsiA) is a chlorophyll-binding membrane-spanning protein in photosynthetic prokaryote cyanobacteria, and is associated with photosystem I (PSI) trimer cores, but its structural and functional significance in light harvesting remains unclear. Here we report a 2.7-Å resolution cryo-electron microscopic structure of a supercomplex between PSI core trimer and IsiA from a thermophilic cyanobacterium Thermosynechococcus vulcanus. The structure showed that 18 IsiA subunits form a closed ring surrounding a PSI trimer core. Detailed arrangement of pigments within the supercomplex, as well as molecular interactions between PSI and IsiA and among IsiAs, were resolved. Time-resolved fluorescence spectra of the PSI-IsiA supercomplex showed clear excitation-energy transfer from IsiA to PSI, strongly indicating that IsiA functions as an energy donor, but not an energy quencher, in the supercomplex. These structural and spectroscopic findings provide important insights into the excitation-energy-transfer and subunit assembly mechanisms in the PSI-IsiA supercomplex.
Identifiants
pubmed: 32393811
doi: 10.1038/s42003-020-0949-6
pii: 10.1038/s42003-020-0949-6
pmc: PMC7214436
doi:
Substances chimiques
Bacterial Proteins
0
Light-Harvesting Protein Complexes
0
Photosystem I Protein Complex
0
chlorophyll A binding protein CP43, Cyanobacteria
148266-13-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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