Evaluating the Effect of Integra Seeded with Adipose Tissue-Derived Stem Cells or Fibroblasts in Wound Healing.
Adipose Tissue
/ cytology
Animals
Burns
/ surgery
Cell Differentiation
Cell Proliferation
Cells, Cultured
Coculture Techniques
Disease Models, Animal
Fibroblasts
/ physiology
Guided Tissue Regeneration
/ methods
Humans
Male
Mice
Primary Cell Culture
Skin
/ cytology
Skin, Artificial
Stem Cells
/ physiology
Wound Healing
/ physiology
Adipose tissue-derived stem cells
bioengineered
fibroblasts
infection
integra
wound healing
Journal
Current drug delivery
ISSN: 1875-5704
Titre abrégé: Curr Drug Deliv
Pays: United Arab Emirates
ID NLM: 101208455
Informations de publication
Date de publication:
2020
2020
Historique:
received:
06
12
2019
revised:
27
02
2020
accepted:
18
04
2020
pubmed:
13
5
2020
medline:
6
7
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
Extensive loss of skin in burn patients can have devastating consequences, both physically and mentally. Adipose-Derived Stem Cells (ADSCs) and fibroblasts are known to play significant roles in the process of wound healing. Recently, bioengineered skin has been considered for wound healing purposes. Investigate the effect of Integra seeded with ADSCs, fibroblasts, or both on wound healing. We found that when Integra is seeded with ADSCs and fibroblasts, both types of cells incorporate and proliferate, the phenomenon becoming more robust when the cells are co-cultured on Integra, both in vitro; and in vivo;. In addition, when these cells are seeded on Integra, they stimulate epithelization with no signs of inflammation and skin necrosis being observed when transplanted on animals for 7 days. ADSCs and fibroblasts seeded on Integra could decrease the number of α-SMA positive myofibroblasts, leading to scarless wound healing. The evidence from this study is strongly supportive that Integra seeded with ADSCs and fibroblasts is an appropriate and effective bioengineered skin for wound healing.
Sections du résumé
BACKGROUND
BACKGROUND
Extensive loss of skin in burn patients can have devastating consequences, both physically and mentally. Adipose-Derived Stem Cells (ADSCs) and fibroblasts are known to play significant roles in the process of wound healing. Recently, bioengineered skin has been considered for wound healing purposes.
METHODS
METHODS
Investigate the effect of Integra seeded with ADSCs, fibroblasts, or both on wound healing.
RESULTS
RESULTS
We found that when Integra is seeded with ADSCs and fibroblasts, both types of cells incorporate and proliferate, the phenomenon becoming more robust when the cells are co-cultured on Integra, both in vitro; and in vivo;. In addition, when these cells are seeded on Integra, they stimulate epithelization with no signs of inflammation and skin necrosis being observed when transplanted on animals for 7 days.
CONCLUSION
CONCLUSIONS
ADSCs and fibroblasts seeded on Integra could decrease the number of α-SMA positive myofibroblasts, leading to scarless wound healing. The evidence from this study is strongly supportive that Integra seeded with ADSCs and fibroblasts is an appropriate and effective bioengineered skin for wound healing.
Identifiants
pubmed: 32394832
pii: CDD-EPUB-106558
doi: 10.2174/1567201817666200512104004
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
629-635Informations de copyright
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