Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat.
Animals
Anti-Allergic Agents
/ isolation & purification
Asarum
/ chemistry
Behavior, Animal
/ drug effects
Cytokines
/ blood
Disease Models, Animal
Drugs, Chinese Herbal
/ isolation & purification
Histamine
/ blood
Immunoglobulin E
/ blood
Male
Nasal Mucosa
/ drug effects
Oils, Volatile
/ isolation & purification
Ovalbumin
Plant Oils
/ isolation & purification
Rats, Sprague-Dawley
Rhinitis, Allergic
/ blood
Nasal mucosa
cytokines
histamine
nasal scratching
sneezing
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
26 06 2020
26 06 2020
Historique:
received:
07
05
2019
revised:
24
04
2020
accepted:
07
05
2020
pubmed:
13
5
2020
medline:
31
3
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
The study was aimed to investigate the protective effect of Asarum sieboldii Miq. essential oil (AEO) on ovalbumin (OVA)-induced allergic rhinitis (AR) in rats. Sixty Sprague-Dawley male rats were randomly divided into six groups (n=10): control, model, cetirizine (Cet, 4.65 g/kg), and AEO (0.5, 1.5, 3 g/kg) groups. All animals except the control group received repeated intranasal instillation with 20 μl of 20% OVA in Al(OH)3 saline solvent for 15 days. The control group was intranasally instilled with 5 mg/ml of Al(OH)3 instead of the same procedure. In the 15 days, Cet and AEO were orally administrated for 28 days. At the end of the drug administration, 20 μl of 5% OVA was given to animals to stimulate allergic reaction, then the rat behavioral detection, assessment of the patho-morphological changes in nasal mucosa, and the serum biomarkers were determined. The result showed that AEO could significantly reduce the amount of nasal secretions, sneezing, and the degree of nasal scratching in AR rats with EC50 = 1.5 and 2.8 g/kg, respectively. The degree of nasal mucosal inflammation in AEO group improved, the levels of immunoglobulin E (IgE), histamine, IL-4, IL-5, IL-17 were decreased, and the level of IFN-γ was increased obviously with EC50 = 2 g/kg. The study suggested that the possible mechanism might be related with the inhibition of histamine release and regulation of the cytokine levels, which plays an important role in the treatment of AR.
Sections du résumé
BACKGROUND
The study was aimed to investigate the protective effect of Asarum sieboldii Miq. essential oil (AEO) on ovalbumin (OVA)-induced allergic rhinitis (AR) in rats.
METHODS AND RESULTS
Sixty Sprague-Dawley male rats were randomly divided into six groups (n=10): control, model, cetirizine (Cet, 4.65 g/kg), and AEO (0.5, 1.5, 3 g/kg) groups. All animals except the control group received repeated intranasal instillation with 20 μl of 20% OVA in Al(OH)3 saline solvent for 15 days. The control group was intranasally instilled with 5 mg/ml of Al(OH)3 instead of the same procedure. In the 15 days, Cet and AEO were orally administrated for 28 days. At the end of the drug administration, 20 μl of 5% OVA was given to animals to stimulate allergic reaction, then the rat behavioral detection, assessment of the patho-morphological changes in nasal mucosa, and the serum biomarkers were determined. The result showed that AEO could significantly reduce the amount of nasal secretions, sneezing, and the degree of nasal scratching in AR rats with EC50 = 1.5 and 2.8 g/kg, respectively. The degree of nasal mucosal inflammation in AEO group improved, the levels of immunoglobulin E (IgE), histamine, IL-4, IL-5, IL-17 were decreased, and the level of IFN-γ was increased obviously with EC50 = 2 g/kg.
CONCLUSION
The study suggested that the possible mechanism might be related with the inhibition of histamine release and regulation of the cytokine levels, which plays an important role in the treatment of AR.
Identifiants
pubmed: 32395767
pii: 224117
doi: 10.1042/BSR20191370
pmc: PMC7268255
pii:
doi:
Substances chimiques
Anti-Allergic Agents
0
Cytokines
0
Drugs, Chinese Herbal
0
Oils, Volatile
0
Plant Oils
0
Immunoglobulin E
37341-29-0
asarum oil
8016-69-1
Histamine
820484N8I3
Ovalbumin
9006-59-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : ExpressionOfConcernIn
Informations de copyright
© 2020 The Author(s).
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