Identification of the minimum region of Bordetella pertussis Vag8 required for interaction with C1 inhibitor.
Bordetella pertussis
C1 inhibitor
Vag8
Journal
Microbiology and immunology
ISSN: 1348-0421
Titre abrégé: Microbiol Immunol
Pays: Australia
ID NLM: 7703966
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
17
03
2020
revised:
06
05
2020
accepted:
10
05
2020
pubmed:
13
5
2020
medline:
16
4
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
An autotransporter of Bordetella pertussis, virulence-associated gene 8 (Vag8), binds and inactivates the complement regulator, C1 inhibitor (C1-Inh), and plays a role in evasion of the complement system. However, the molecular interaction between Vag8 and C1-Inh remains unclear. Here, we localized the minimum region of Vag8 required for interaction with C1-Inh by examining the differently truncated Vag8 derivatives for the ability to bind and inactivate C1-Inh. The truncated Vag8 containing amino-acid residues 102-548, but not 102-479 and 202-648, showed the full activity of intact Vag8, suggesting that the separate 102-202 and 548-648 amino-acid regions of Vag8 mediate the interaction with C1-Inh.
Identifiants
pubmed: 32396237
doi: 10.1111/1348-0421.12799
pmc: PMC7497153
doi:
Substances chimiques
Bacterial Proteins
0
Complement C1 Inhibitor Protein
0
SERPING1 protein, human
0
Type V Secretion Systems
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
570-573Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17H04075
Organisme : Japan Society for the Promotion of Science
ID : 19K16638
Informations de copyright
© 2020 The Authors. Microbiology and Immunology published by The Societies and John Wiley & Sons Australia, Ltd.
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