Spleen and thymus metabolomics strategy to explore the immunoregulatory mechanism of total withanolides from the leaves of Datura metel L. on imiquimod-induced psoriatic skin dermatitis in mice.
Animals
Datura metel
/ chemistry
Disease Models, Animal
Imiquimod
/ adverse effects
Immunosuppressive Agents
/ pharmacology
Male
Metabolome
/ drug effects
Metabolomics
Mice
Mice, Inbred C57BL
Plant Extracts
/ chemistry
Plant Leaves
/ chemistry
Psoriasis
/ chemically induced
Spleen
/ drug effects
Thymus Gland
/ drug effects
Withanolides
/ pharmacology
Datura metel L
metabolomics
psoriasis
spleen
thymus
withanolides
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
26
12
2019
revised:
24
04
2020
accepted:
05
05
2020
pubmed:
13
5
2020
medline:
1
4
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
Our previous work demonstrated that total withanolides of Datura metel L. leaves (TWD) exhibited excellent therapeutic effects on psoriasis. However, current knowledge of its mechanisms is incomplete. In this study, integrated spleen and thymus untargeted metabolomics were used to analyze the changes in endogenous metabolites underlying the immunosuppressive activity of TWD on psoriasis animal models induced by imiquimod. The results suggested that TWD treatment markedly attenuated imiquimod-induced psoriasis and showed significant immunosuppressive activity as evidenced by decreased elevation index of spleen and thymus. Meanwhile, TWD significantly reversed the elevation of immunoregulatory factors, including IL-10, IL-17, IL-22 and IL-23. Multivariate trajectory analysis revealed that TWD treatment could restore the psoriasis-disturbed spleen and thymus metabolite profiles towards the normal metabolic status. A total of 25 and 27 metabolites associated with the immunomodulatory effects for which levels changed markedly upon treatment have been identified in spleen and thymus, respectively. These differential metabolites were mainly involved in amino acid metabolism, nucleotide metabolism, fatty acid metabolism and lipid metabolism. Our investigation provided a holistic view of TWD for intervention in psoriasis through immunoregulation and provided further scientific information in vivo about a clinical value of TWD for psoriasis.
Substances chimiques
Immunosuppressive Agents
0
Plant Extracts
0
Withanolides
0
Imiquimod
P1QW714R7M
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4881Subventions
Organisme : China Postdoctoral Science Foundation
ID : 2018M631978
Organisme : Heilongjiang university of Chinese medicine Founds
ID : 2018bs03
Organisme : Heilongjiang university of Chinese medicine Founds
ID : 2018pt01
Organisme : Heilongjiang Postdoctoral Science Foundation
ID : LBH-Z18245
Organisme : Excellent Youth Project of Heilongjiang Natural Science Foundation
ID : YQ2019H029
Organisme : National Natural Science Foundation of China
ID : 81773883
Organisme : National Natural Science Foundation of China
ID : 81903781
Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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