Automated high-throughput analysis of tramadol and O-desmethyltramadol in dried blood spots.
Automation
Chromatography, Liquid
/ methods
Doping in Sports
/ prevention & control
Dried Blood Spot Testing
/ methods
Female
High-Throughput Screening Assays
/ methods
Humans
Male
Pilot Projects
Reproducibility of Results
Substance Abuse Detection
/ methods
Tandem Mass Spectrometry
/ methods
Tramadol
/ analogs & derivatives
antidoping
dried blood spots
fully automated analysis
tramadol
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
17
04
2020
revised:
30
04
2020
accepted:
08
05
2020
pubmed:
13
5
2020
medline:
29
6
2021
entrez:
13
5
2020
Statut:
ppublish
Résumé
The World Anti-Doping Agency (WADA) and the International Testing Agency (ITA) recently announced the development and implementation of dried blood spot (DBS) testing for routine analysis in time for the 2022 Winter Olympic and Paralympic Games in Beijing. Following the introduction of a ban on the use of tramadol in competition in March 2019, the Union Cycliste International (UCI) started a pilot study for the manual analysis of tramadol in DBS for antidoping purposes. In this context, we present a fully automated LC-MS/MS-based method with automated sample preparation using a CAMAG DBS-MS 500 for the analysis of tramadol and its metabolite O-desmethyltramadol in DBS. The presented approach reduces manual handling in the laboratory to an absolute minimum, only requiring the preparation of calibration and quality control DBS cards. The method was developed, optimized, and validated before performing cross-validation with a liquid blood-based analysis method using authentic samples from forensic cases. During the validation process, the method showed an extraction efficiency of 62%, linearity r
Substances chimiques
O-demethyltramadol
2WA8F50C3F
Tramadol
39J1LGJ30J
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1126-1134Informations de copyright
© 2020. John Wiley & Sons, Ltd.
Références
Baltazar-Martins G, Plata M del M, Muñoz-Guerra J, Muñoz G, Carreras D, Del Coso J. Prevalence of tramadol findings in urine samples obtained in competition. Drug Test Anal. 2019;11:631-4. https://doi.org/10.1002/dta.2575
Pennings E, Bodenschatz C, Beran D. WHO Tramadol Update Review Report Agenda Item 6.1.; 2014.
Goeringer KE, Logan BK, Christian GD. Identification of tramadol and its metabolites in blood from drug-related deaths and drug-impaired drivers. J Anal Toxicol. 1997;21(7):529-537. https://doi.org/10.1093/jat/21.7.529
Prince A ( National PIS. (Newcastle C. Summary for UKPID - Tramadol. Tramadol. http://www.inchem.org/documents/ukpids/ukpids/ukpid17.htm. Published 1997. Accessed April 1, 2020.
Clarot F, Goullé JP, Vaz E, Proust B. Fatal overdoses of tramadol: is benzodiazepine a risk factor of lethality? Forensic Sci Int. 2003;134(1):57-61. https://doi.org/10.1016/S0379-0738(03)00100-2
Internationale UC. Inside UCI - Tramadol ban: All you need to know. https://www.uci.org/inside-uci/press-releases/tramadol-ban-all-you-need-to-know. Published 2019. .
Luginbühl M, Gaugler S. The application of fully automated dried blood spot analysis for liquid chromatography-tandem mass spectrometry using the CAMAG DBS-MS 500 autosampler. Clin Biochem. February 2020. https://doi.org/10.1016/j.clinbiochem.2020.02.007
Velghe S, Delahaye L, Stove CP. Is the hematocrit still an issue in quantitative dried blood spot analysis? J Pharm Biomed Anal. 2019;163:188-196. https://doi.org/10.1016/j.jpba.2018.10.010
US-FDA. Bioanalytical Method Validation: Guidance for Industry.; 2018.
Khan FY, Yousef H, Errayes M. Tramadol toxicity-induced rhabdomyolysis. J Emerg Trauma Shock. 2010;3:421-422. https://doi.org/10.4103/0974-2700.70766
Union Cycliste Internationale. Misuse of tramadol: UCI report on tests carried out during the first part of the 2019 season. Inside UCI. https://www.uci.org/inside-uci/press-releases/misuse-of-tramadol-uci-report-on-tests-carried-out-during-the-first-part-of-the-2019-season. Published 2019. Accessed 10 April 2020.
Abu-Rabie P, Denniff P, Spooner N, Chowdhry BZ, Pullen FS. Investigation of different approaches to incorporating internal standard in DBS quantitative bioanalytical workflows and their effect on nullifying hematocrit-based assay bias. Anal Chem. 2015;87(9):4996-5003. https://doi.org/10.1021/acs.analchem.5b00908