A phylogenetic analysis of the biting midges belonging to Culicoides Latreille (Diptera: Ceratopogonidae) subgenus Avaritia using molecular data.
Culicoides
phylogeny
species group
systematics
taxonomy
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
12 May 2020
12 May 2020
Historique:
received:
22
09
2019
accepted:
29
04
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
29
1
2021
Statut:
epublish
Résumé
Within the genus Culicoides (Diptera: Ceratopogonidae), the subgenus Avaritia is of particular interest as it contains a significant number of economically important vector species. Disagreements about the systematic classification of species within this subgenus have resulted in a taxonomic imbroglio. A molecular phylogeny of the subgenus Avaritia was conducted to test the existing systematic classification, which is based on phenetic assessment of morphological characters. Three nuclear ribosomal markers, internal transcribed spacer 1 and 2 (ITS1, ITS2), 5.8S, and three mitochondrial markers, cytochrome c oxidase subunit 1 and 2, and cytochrome b (cox1, cox2 and cytb), were obtained for 37 species of the subgenus Avaritia from all six biogeographical regions. Phylogenetic reconstructions using these genes independently and in combination were implemented using Bayesian inference analysis and maximum likelihood methods. Phylogenetic reconstructions gave strong support to several monophyletic groups within the subgenus Avaritia. Both C. actoni and C. pusillus formed a single clade with C. grahamii so their respective groups, the Actoni and Pusillus groups, have been merged with the Grahamii group. Some support was provided for the Boophagus and Jacobsoni groups. A group of species currently placed into the Orientalis group clustered in a clade with poor support. The Obsoletus group was defined as a sister clade to all other Avaritia groups. The clade including the Imicola group was well supported based on phylogenetic criteria. This phylogenetic study combining five distinct molecular markers has provided meaningful insights into the systematic relationships of Culicoides (Avaritia) and highlighted future directions to continue the study of this subgenus. While the cox2 marker appeared to be useful to investigate closely related species, the 5.8S marker was highly conserved and uninformative. Further investigations including species absent from this work are needed to confirm the proposed systematic scheme. However, this systematic scheme can now serve as a foundation to investigate cryptic species affiliation within the subgenus. We advocate that future studies employ a combination of morphological and molecular analyses.
Sections du résumé
BACKGROUND
BACKGROUND
Within the genus Culicoides (Diptera: Ceratopogonidae), the subgenus Avaritia is of particular interest as it contains a significant number of economically important vector species. Disagreements about the systematic classification of species within this subgenus have resulted in a taxonomic imbroglio.
METHODS
METHODS
A molecular phylogeny of the subgenus Avaritia was conducted to test the existing systematic classification, which is based on phenetic assessment of morphological characters. Three nuclear ribosomal markers, internal transcribed spacer 1 and 2 (ITS1, ITS2), 5.8S, and three mitochondrial markers, cytochrome c oxidase subunit 1 and 2, and cytochrome b (cox1, cox2 and cytb), were obtained for 37 species of the subgenus Avaritia from all six biogeographical regions. Phylogenetic reconstructions using these genes independently and in combination were implemented using Bayesian inference analysis and maximum likelihood methods.
RESULTS
RESULTS
Phylogenetic reconstructions gave strong support to several monophyletic groups within the subgenus Avaritia. Both C. actoni and C. pusillus formed a single clade with C. grahamii so their respective groups, the Actoni and Pusillus groups, have been merged with the Grahamii group. Some support was provided for the Boophagus and Jacobsoni groups. A group of species currently placed into the Orientalis group clustered in a clade with poor support. The Obsoletus group was defined as a sister clade to all other Avaritia groups. The clade including the Imicola group was well supported based on phylogenetic criteria.
CONCLUSIONS
CONCLUSIONS
This phylogenetic study combining five distinct molecular markers has provided meaningful insights into the systematic relationships of Culicoides (Avaritia) and highlighted future directions to continue the study of this subgenus. While the cox2 marker appeared to be useful to investigate closely related species, the 5.8S marker was highly conserved and uninformative. Further investigations including species absent from this work are needed to confirm the proposed systematic scheme. However, this systematic scheme can now serve as a foundation to investigate cryptic species affiliation within the subgenus. We advocate that future studies employ a combination of morphological and molecular analyses.
Identifiants
pubmed: 32398143
doi: 10.1186/s13071-020-04111-4
pii: 10.1186/s13071-020-04111-4
pmc: PMC7216621
doi:
Substances chimiques
Cytochrome b Group
0
DNA, Intergenic
0
Electron Transport Complex IV
EC 1.9.3.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
243Références
PLoS One. 2019 Nov 7;14(11):e0224599
pubmed: 31697735
Annu Rev Entomol. 2015 Jan 7;60:373-92
pubmed: 25386725
Trends Microbiol. 2009 Apr;17(4):172-8
pubmed: 19299131
Mol Biol Evol. 2008 Jul;25(7):1253-6
pubmed: 18397919
Infect Genet Evol. 2015 Mar;30:249-266
pubmed: 25535946
Parasite. 2017;24:23
pubmed: 28643630
Parasit Vectors. 2014 Sep 30;7:445
pubmed: 25270724
Epidemiol Infect. 2013 Jan;141(1):1-8
pubmed: 23046921
Mol Biol Evol. 2011 Oct;28(10):2731-9
pubmed: 21546353
J Med Entomol. 2007 Nov;44(6):1019-25
pubmed: 18047201
Natl Inst Anim Health Q (Tokyo). 1980 Spring;20(1):11-22
pubmed: 7432546
Bioinformatics. 2003 Aug 12;19(12):1572-4
pubmed: 12912839
Med Vet Entomol. 2006 Jun;20(2):229-38
pubmed: 16796616
Syst Biol. 2005 Jun;54(3):373-90
pubmed: 16012105
J Med Entomol. 2009 Sep;46(5):1099-108
pubmed: 19769041
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
pubmed: 7984417
Mol Biol Evol. 2010 Feb;27(2):221-4
pubmed: 19854763
Zookeys. 2017 Oct 4;(706):117-135
pubmed: 29118624
Methods Mol Biol. 2009;537:39-64
pubmed: 19378139
Zootaxa. 2014 Feb 27;3768:401-27
pubmed: 24871184
Acta Trop. 2015 Feb;142:5-19
pubmed: 25447828
Onderstepoort J Vet Res. 1998 Mar;65(1):9-16
pubmed: 9629585
Vet Ital. 2004 Jul-Sep;40(3):151-9
pubmed: 20419654
Vet Parasitol. 2012 Mar 23;184(2-4):258-66
pubmed: 21963364
Mol Phylogenet Evol. 2020 Apr;145:106736
pubmed: 31978488
Med Vet Entomol. 2002 Jun;16(2):139-46
pubmed: 12109707
Vet Ital. 2015 Oct-Dec;51(4):345-78
pubmed: 26741249
Vet Ital. 2004 Jul-Sep;40(3):345-51
pubmed: 20419691
Med Vet Entomol. 2013 Sep;27(3):323-31
pubmed: 23106166
Syst Biol. 2004 Feb;53(1):47-67
pubmed: 14965900
J Med Entomol. 1990 Mar;27(2):85-195
pubmed: 2093769
Parasit Vectors. 2018 Feb 27;11(1):116
pubmed: 29486789
Natl Inst Anim Health Q (Tokyo). 1973 Winter;13(4):211-19
pubmed: 4792271
Med Vet Entomol. 2006 Jun;20(2):219-28
pubmed: 16796615
Vet Res. 2004 May-Jun;35(3):325-37
pubmed: 15210081
Bull Entomol Res. 2009 Aug;99(4):371-5
pubmed: 19063759
Med Vet Entomol. 2003 Dec;17(4):379-87
pubmed: 14651651
J Hered. 2001 Jul-Aug;92(4):371-3
pubmed: 11535656
Med Vet Entomol. 2009 Mar;23(1):47-55
pubmed: 19239613
Syst Biol. 2010 May;59(3):307-21
pubmed: 20525638