Testing Albendazole Resistance in Fasciola hepatica.
Albendazole
Diagnostic
Egg development test
Fasciola hepatica
Ovicidal activity
Resistance
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
12
3
2021
Statut:
ppublish
Résumé
The egg development test is a useful in vitro tool to detect albendazole (ABZ) resistance in Fasciola hepatica. ABZ is the only flukicidal compound with ovicidal activity. The described test is based on the ABZ capacity to affect parasite egg development and hatching in susceptible parasites, while this effect is lost in ABZ-resistant liver fluke isolates. Among many advantages, it is noted that the diagnostic test can be performed on eggs isolated from fecal samples (sheep and cattle), avoiding the sacrifice of animals necessary in controlled efficacy trials. The egg development test described here is a simple, inexpensive, and accessible method, previously employed for diagnosis of ABZ resistance in F. hepatica.
Identifiants
pubmed: 32399932
doi: 10.1007/978-1-0716-0475-5_16
doi:
Substances chimiques
Albendazole
F4216019LN
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
213-220Références
McKellar Q, Scott E (1990) The benzimidazole anthelmintic agents: a review. J Vet Pharmacol Ther 13:223–247
doi: 10.1111/j.1365-2885.1990.tb00773.x
Álvarez-Sánchez MA, Mainar-Jaime RC, Pérez-García J et al (2006) Resistance of Fasciola hepatica to triclabendazole and albendazole in sheep in Spain. Vet Rec 159:424–425
doi: 10.1136/vr.159.13.424
Sanabria R, Ceballos L, Moreno L et al (2013) Identification of a field isolate of Fasciola hepatica resistant to albendazole and susceptible to triclabendazole. Vet Parasitol 193:105–110
doi: 10.1016/j.vetpar.2012.11.033
Novobilsky A, Amaya Solis N, Skarin M et al (2016) Assessment of flukicide efficacy against Fasciola hepatica in sheep in Sweden in the absence of a standardised test. Int J Parasitol Drugs Drug Resist 6:141–147
doi: 10.1016/j.ijpddr.2016.06.004
Wood IB, Amaral NK, Bairden K et al (1995) World Association for the Advancement of veterinary parasitology (W.A.A.V.P.) second edition of guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Vet Parasitol 58:181–213
doi: 10.1016/0304-4017(95)00806-2
Fairweather I (2011) Reducing the future threat from (liver) fluke: realistic prospect or quixotic fantasy? Vet Parasitol 180:133–143
doi: 10.1016/j.vetpar.2011.05.034
Lacey E (1990) Mode of action of benzimidazoles. Parasitol Today 6:112–115
doi: 10.1016/0169-4758(90)90227-U
Coles GC, Bauer C, Borgsteede F et al (1992) World Association for the Advancement of veterinary parasitology (W.A.A.V.P.) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 44:35–44
doi: 10.1016/0304-4017(92)90141-U
Alvarez L, Moreno G, Moreno L et al (2009) Comparative assessment of albendazole and triclabendazole ovicidal activity on Fasciola hepatica eggs. Vet Parasitol 164:211–216
doi: 10.1016/j.vetpar.2009.05.014
Canevari J, Ceballos L, Sanabria R et al (2014) Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom. J Helminthol 88:286–292
doi: 10.1017/S0022149X13000163
Robles-Pérez D, Martínez-Pérez JM, Rojo-Vázquez FA (2014) Development of an egg hatch assay for the detection of anthelmintic resistance to albendazole in Fasciola hepatica isolated from sheep. Vet Parasitol 203:217–221
doi: 10.1016/j.vetpar.2013.11.020