Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish.
Aging
/ genetics
Animals
Animals, Genetically Modified
Disease Models, Animal
Electrocardiography
Humans
Mice
Models, Cardiovascular
Mutation, Missense
NAV1.5 Voltage-Gated Sodium Channel
/ genetics
Sick Sinus Syndrome
/ etiology
Sinus Arrest, Cardiac
/ etiology
Species Specificity
Zebrafish
/ genetics
Zebrafish Proteins
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
02
2020
accepted:
15
04
2020
entrez:
14
5
2020
pubmed:
14
5
2020
medline:
1
8
2020
Statut:
epublish
Résumé
Because of its powerful genetics, the adult zebrafish has been increasingly used for studying cardiovascular diseases. Considering its heart rate of ~100 beats per minute at ambient temperature, which is very close to human, we assessed the use of this vertebrate animal for modeling heart rhythm disorders such as sinus arrest (SA) and sick sinus syndrome (SSS). We firstly optimized a protocol to measure electrocardiogram in adult zebrafish. We determined the location of the probes, implemented an open-chest microsurgery procedure, measured the effects of temperature, and determined appropriate anesthesia dose and time. We then proposed an PP interval of more than 1.5 seconds as an arbitrary criterion to define an SA episode in an adult fish at ambient temperature, based on comparison between the current definition of an SA episode in humans and our studies of candidate SA episodes in aged wild-type fish and Tg(SCN5A-D1275N) fish (a fish model for inherited SSS). With this criterion, a subpopulation of about 5% wild-type fish can be considered to have SA episodes, and this percentage significantly increases to about 25% in 3-year-old fish. In response to atropine, this subpopulation has both common SSS phenotypic traits that are shared with the Tg(SCN5A-D1275N) model, such as bradycardia; and unique SSS phenotypic traits, such as increased QRS/P ratio and chronotropic incompetence. In summary, this study defined baseline SA and SSS in adult zebrafish and underscored use of the zebrafish as an alternative model to study aging-associated SSS.
Identifiants
pubmed: 32401822
doi: 10.1371/journal.pone.0232457
pii: PONE-D-20-03005
pmc: PMC7219707
doi:
Substances chimiques
NAV1.5 Voltage-Gated Sodium Channel
0
Zebrafish Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0232457Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL081753
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL107304
Pays : United States
Organisme : NIH HHS
ID : R41 OD024874
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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