MNase Profiling of Promoter Chromatin in
GM12878
Salmonella typhimurium
chromatin
micrococcal nuclease
nucleosome
Journal
G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598
Informations de publication
Date de publication:
07 07 2020
07 07 2020
Historique:
pubmed:
15
5
2020
medline:
22
6
2021
entrez:
15
5
2020
Statut:
epublish
Résumé
The nucleosome is the primary unit of chromatin structure and commonly imputed as a regulator of nuclear events, although the exact mechanisms remain unclear. Recent studies have shown that certain nucleosomes can have different sensitivities to micrococcal nuclease (MNase) digestion, resulting in the release of populations of nucleosomes dependent on the concentration of MNase. Mapping MNase sensitivity of nucleosomes at transcription start sites genome-wide reveals an important functional nucleosome organization that correlates with gene expression levels and transcription factor binding. In order to understand nucleosome distribution and sensitivity dynamics during a robust genome response, we mapped nucleosome position and sensitivity using multiple concentrations of MNase. We used the innate immune response as a model system to understand chromatin-mediated regulation. Herein we demonstrate that stimulation of a human lymphoblastoid cell line (GM12878) with heat-killed
Identifiants
pubmed: 32404364
pii: g3.120.401266
doi: 10.1534/g3.120.401266
pmc: PMC7341138
doi:
Substances chimiques
Chromatin
0
Nucleosomes
0
Micrococcal Nuclease
EC 3.1.31.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2171-2178Informations de copyright
Copyright © 2020 Lauren and Jonathan.
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