Reconstitution reveals two paths of force transmission through the kinetochore.
Chromosome Segregation
Chromosomes, Fungal
DNA-Binding Proteins
/ genetics
Kinetochores
/ metabolism
Mechanotransduction, Cellular
Microtubules
/ genetics
Nuclear Proteins
/ genetics
Nucleosomes
/ genetics
Saccharomyces cerevisiae
/ genetics
Saccharomyces cerevisiae Proteins
/ genetics
Stress, Mechanical
E. coli
S. cerevisiae
biochemistry
cell biology
centromere
chemical biology
chromosome segregation
kinetochore
mitosis
optical trap
optical tweezers
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
14 05 2020
14 05 2020
Historique:
received:
03
03
2020
accepted:
13
05
2020
pubmed:
15
5
2020
medline:
18
3
2021
entrez:
15
5
2020
Statut:
epublish
Résumé
Partitioning duplicated chromosomes equally between daughter cells is a microtubule-mediated process essential to eukaryotic life. A multi-protein machine, the kinetochore, drives chromosome segregation by coupling the chromosomes to dynamic microtubule tips, even as the tips grow and shrink through the gain and loss of subunits. The kinetochore must harness, transmit, and sense mitotic forces, as a lack of tension signals incorrect chromosome-microtubule attachment and precipitates error correction mechanisms. But though the field has arrived at a 'parts list' of dozens of kinetochore proteins organized into subcomplexes, the path of force transmission through these components has remained unclear. Here we report reconstitution of functional
Identifiants
pubmed: 32406818
doi: 10.7554/eLife.56582
pii: 56582
pmc: PMC7367685
doi:
pii:
Substances chimiques
DNA-Binding Proteins
0
MIF2 protein, S cerevisiae
0
NDC80 protein, S cerevisiae
0
Nuclear Proteins
0
Nucleosomes
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM079373
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM040506
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130293
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134842
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008268
Pays : United States
Informations de copyright
© 2020, Hamilton et al.
Déclaration de conflit d'intérêts
GH, LH, CA, TD No competing interests declared, CN, YD is affiliated with Genentech Inc. The author has no financial interests to declare
Références
J Cell Biol. 1994 Sep;126(5):1241-53
pubmed: 8063861
Protein Expr Purif. 2003 Nov;32(1):83-8
pubmed: 14680943
J Cell Biol. 2014 Aug 18;206(4):509-24
pubmed: 25135934
Nat Cell Biol. 2012 May 06;14(6):604-13
pubmed: 22561346
Annu Rev Biochem. 2004;73:705-48
pubmed: 15189157
Nat Cell Biol. 2012 May 06;14(6):614-24
pubmed: 22561345
Elife. 2018 Apr 09;7:
pubmed: 29629870
Biochim Biophys Acta. 2008 Sep;1786(1):32-40
pubmed: 18549824
Nat Rev Mol Cell Biol. 2015 Aug;16(8):473-85
pubmed: 26204159
Genes Dev. 2003 Dec 1;17(23):2902-21
pubmed: 14633972
Methods. 2010 Jun;51(2):242-50
pubmed: 20096784
Elife. 2019 May 21;8:
pubmed: 31112132
EMBO J. 2019 Jan 3;38(1):
pubmed: 30389668
Genes Dev. 1999 May 1;13(9):1140-55
pubmed: 10323865
EMBO J. 2013 Feb 6;32(3):424-36
pubmed: 23334297
Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2740-2745
pubmed: 29487209
Nature. 2011 Aug 28;477(7364):354-8
pubmed: 21874020
Genes Dev. 2017 Oct 1;31(19):1958-1972
pubmed: 29074736
Biology (Basel). 2017 Jan 24;6(1):
pubmed: 28125021
Structure. 2020 Mar 3;28(3):363-370.e3
pubmed: 32004465
Cell. 2002 Oct 18;111(2):163-72
pubmed: 12408861
Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5363-7
pubmed: 15809444
Curr Biol. 2011 Mar 8;21(5):391-8
pubmed: 21353556
Chromosome Res. 2014 Sep;22(3):293-303
pubmed: 24481920
Nat Cell Biol. 2008 Apr;10(4):407-14
pubmed: 18364702
Mol Cell. 2018 Sep 20;71(6):923-939.e10
pubmed: 30174292
Science. 2013 May 31;340(6136):1110-3
pubmed: 23723239
Nature. 2016 Sep 8;537(7619):249-253
pubmed: 27580032
Nat Cell Biol. 2006 Jun;8(6):581-5
pubmed: 16715078
Open Biol. 2016 Feb;6(2):150236
pubmed: 26911624
Biophys J. 1997 Apr;72(4):1541-55
pubmed: 9083660
Curr Biol. 2020 Feb 24;30(4):561-572.e10
pubmed: 32032508
Cell. 2006 Dec 1;127(5):983-97
pubmed: 17129783
Cell. 2009 Mar 6;136(5):865-75
pubmed: 19269365
Nat Commun. 2014 Sep 19;5:4951
pubmed: 25236177
Elife. 2014 Sep 23;3:
pubmed: 25247700
Elife. 2017 Feb 13;6:
pubmed: 28191870
Cell. 2011 Apr 29;145(3):410-22
pubmed: 21529714
Cell. 2016 Nov 3;167(4):1014-1027.e12
pubmed: 27881300
Prog Mol Subcell Biol. 2017;56:29-57
pubmed: 28840232
Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):E5583-9
pubmed: 26430240
Elife. 2018 Aug 17;7:
pubmed: 30117803
Methods Cell Biol. 2010;95:641-56
pubmed: 20466157
Cell. 1986 Jan 17;44(1):53-63
pubmed: 3510080
Nature. 2010 Nov 25;468(7323):576-9
pubmed: 21107429
Cell. 2016 Nov 3;167(4):1028-1040.e15
pubmed: 27881301
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9250-5
pubmed: 19478070
Proc Natl Acad Sci U S A. 1991 May 1;88(9):3734-8
pubmed: 2023923
J Cell Biol. 2013 Dec 23;203(6):957-69
pubmed: 24344188
Elife. 2016 Dec 24;5:
pubmed: 28012276
Philos Trans R Soc Lond B Biol Sci. 2011 Dec 27;366(1584):3595-604
pubmed: 22084386
Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9873-8
pubmed: 16777964
Nature. 2002 Jul 25;418(6896):387-91
pubmed: 12140549
J Cell Biol. 2001 Apr 30;153(3):517-27
pubmed: 11331303
J Cell Biol. 2010 Jun 28;189(7):1143-55
pubmed: 20566683
J Cell Biol. 2011 Nov 14;195(4):573-82
pubmed: 22084307
Cell. 2016 Jun 2;165(6):1428-1439
pubmed: 27156448
J Mol Biol. 1998 Feb 13;276(1):19-42
pubmed: 9514715
Cell Rep. 2013 Oct 17;5(1):29-36
pubmed: 24075991
EMBO Rep. 2017 Sep;18(9):1559-1571
pubmed: 28642229
Cell Cycle. 2005 Oct;4(10):1448-56
pubmed: 16177574
J Cell Biol. 2014 May 12;205(3):313-24
pubmed: 24821839
Elife. 2019 May 02;8:
pubmed: 31045495
Nature. 1999 Jan 7;397(6714):50-3
pubmed: 9892352
Genes Dev. 1995 Mar 1;9(5):573-86
pubmed: 7698647
Nat Commun. 2016 Oct 20;7:13221
pubmed: 27762268
Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7282-7
pubmed: 23589891
Nat Cell Biol. 2016 Apr;18(4):382-92
pubmed: 26974660
Annu Rev Biophys Biophys Chem. 1988;17:431-49
pubmed: 3293594
J Cell Biol. 2010 May 17;189(4):713-23
pubmed: 20479468
J Cell Biol. 2017 Nov 6;216(11):3551-3570
pubmed: 28939613