Non-thermal plasma accelerates the healing process of peripheral nerve crush injury in rats.
Animals
Axons
/ physiology
Crush Injuries
/ etiology
Disease Models, Animal
Feasibility Studies
Humans
Male
Myelin Sheath
/ physiology
Nerve Regeneration
/ physiology
Peripheral Nerve Injuries
/ etiology
Plasma Gases
/ therapeutic use
Rats
Recovery of Function
/ physiology
Sciatic Nerve
/ injuries
Time Factors
Macrophage
Myelin Sheath
Neuronal Axon
Non-thermal plasma (NTP)
Sciatic Nerve Crush Injury (SNCI)
Skeletal Muscle Healing
Journal
International journal of medical sciences
ISSN: 1449-1907
Titre abrégé: Int J Med Sci
Pays: Australia
ID NLM: 101213954
Informations de publication
Date de publication:
2020
2020
Historique:
received:
17
01
2020
accepted:
13
04
2020
entrez:
16
5
2020
pubmed:
16
5
2020
medline:
9
3
2021
Statut:
epublish
Résumé
The objective of this study was to evaluate the effect of non-thermal plasma (NTP) on the healing process of peripheral nerve crush injuries, which can occur during dental implant procedures. For this, a rat model of sciatic nerve crush injury (SNCI) was adopted. The rats were divided into three groups: non-nerve damage (non-ND), nerve damage (ND), and ND+NTP group. To evaluate the sciatic nerve (SN) function, the static sciatic index was calculated, and the muscle and SN tissues were subjected to a histologic analysis. The results showed that NTP effectively accelerated the healing process of SNCI in rats. In contrast to the ND group, which showed approximately 60% recovery in the SN function, the NTP-treated rats showed complete recovery. Histologically, the NTP treatments not only accelerated the muscle healing, but also reduced the edema-like phenotype of the damaged SN tissues. In the ND group, the SN tissues had an accumulation of CD68-positive macrophages, partially destroyed axonal fibers and myelinated Schwann cells. Conversely, in the ND+NTP group, the macrophage accumulation was reduced and an overall regeneration of the damaged axon fibers and the myelin sheath was accomplished. The results of this study indicate that NTP can be used for healing of injured peripheral nerves.
Identifiants
pubmed: 32410841
doi: 10.7150/ijms.44041
pii: ijmsv17p1112
pmc: PMC7211154
doi:
Substances chimiques
Plasma Gases
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1112-1120Informations de copyright
© The author(s).
Déclaration de conflit d'intérêts
Competing Interests: The authors have declared that no competing interest exists.
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