Symptoms and immunoglobulin development in hospital staff exposed to a SARS-CoV-2 outbreak.
Adolescent
Adult
Aged
Antibodies, Viral
/ blood
Asymptomatic Diseases
Biomarkers
/ blood
COVID-19
/ diagnosis
COVID-19 Serological Testing
Cross-Sectional Studies
Disease Outbreaks
Enzyme-Linked Immunosorbent Assay
Female
Germany
/ epidemiology
Humans
Immunity, Herd
Immunoglobulin A
/ blood
Immunoglobulin G
/ blood
Male
Middle Aged
Occupational Diseases
/ diagnosis
Occupational Exposure
Personnel, Hospital
SARS-CoV-2
/ immunology
Young Adult
COVID-19 outbreak
COVID-19 pandemic
SARS-CoV-2
antibody response
healthcare workers
immunoglobulin development
infection chain
Journal
Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology
ISSN: 1399-3038
Titre abrégé: Pediatr Allergy Immunol
Pays: England
ID NLM: 9106718
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
30
04
2020
revised:
09
05
2020
accepted:
10
05
2020
pubmed:
16
5
2020
medline:
29
12
2020
entrez:
16
5
2020
Statut:
ppublish
Résumé
Worldwide, the number of SARS-CoV-2 infections is increasing. Serological immunoglobulin tests may help to better understand the development of immune mechanisms against SARS-CoV-2 in COVID-19 cases and exposed but asymptomatic individuals. The aim of this study was to investigate exposure to SARS-CoV-2, symptoms, and antibody responses in a large sample of healthcare workers following a COVID-19 outbreak. A COVID-19 outbreak among staff members of a major German children's and women's hospital was followed by massive RT-PCR SARS-CoV-2 tests and provided the opportunity to study symptoms, chains of infection, and SARS-CoV-2-specific antibody responses (IgG and IgA) by ELISA. Study participants were classified as COVID-19 cases, and persons with close, moderate, or no exposure to SARS-CoV-2 in the clinical setting, respectively. Out of 201 study participants, 31 were COVID-19 cases. While most study participants experienced many symptoms indicative for SARS-CoV-2 infection, anosmia and coughing were remarkably more frequent in COVID-19 cases. Approximately 80% of COVID-19 cases developed some specific antibody response (IgA and IgG) approximately 3 weeks after onset of symptoms. Subjects in the non-COVID-19 groups had also elevated IgG (1.8%) and IgA values (7.6%) irrespective of contact history with cases. We found that a significant number of diseased did not develop relevant antibody responses three weeks after symptom onset. Our data also suggest that exposure to COVID-19 positive co-workers in a hospital setting is not leading to the development of measurable immune responses in a significant proportion of asymptomatic contact persons.
Sections du résumé
BACKGROUND
Worldwide, the number of SARS-CoV-2 infections is increasing. Serological immunoglobulin tests may help to better understand the development of immune mechanisms against SARS-CoV-2 in COVID-19 cases and exposed but asymptomatic individuals. The aim of this study was to investigate exposure to SARS-CoV-2, symptoms, and antibody responses in a large sample of healthcare workers following a COVID-19 outbreak.
METHODS
A COVID-19 outbreak among staff members of a major German children's and women's hospital was followed by massive RT-PCR SARS-CoV-2 tests and provided the opportunity to study symptoms, chains of infection, and SARS-CoV-2-specific antibody responses (IgG and IgA) by ELISA. Study participants were classified as COVID-19 cases, and persons with close, moderate, or no exposure to SARS-CoV-2 in the clinical setting, respectively.
RESULTS
Out of 201 study participants, 31 were COVID-19 cases. While most study participants experienced many symptoms indicative for SARS-CoV-2 infection, anosmia and coughing were remarkably more frequent in COVID-19 cases. Approximately 80% of COVID-19 cases developed some specific antibody response (IgA and IgG) approximately 3 weeks after onset of symptoms. Subjects in the non-COVID-19 groups had also elevated IgG (1.8%) and IgA values (7.6%) irrespective of contact history with cases.
CONCLUSION
We found that a significant number of diseased did not develop relevant antibody responses three weeks after symptom onset. Our data also suggest that exposure to COVID-19 positive co-workers in a hospital setting is not leading to the development of measurable immune responses in a significant proportion of asymptomatic contact persons.
Substances chimiques
Antibodies, Viral
0
Biomarkers
0
Immunoglobulin A
0
Immunoglobulin G
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
841-847Informations de copyright
© 2020 The Authors. Pediatric Allergy and Immunology published by John Wiley & Sons Ltd.
Références
World Health Organization (WHO). Corona virus disease (COVID-19) Pandemic. https://www.who.int/emergencies/diseases/novel-coronavirus-2019. Accessed April 29, 2020.
Okba NMA, Muller MA, Li W, et al. Severe acute respiratory syndrome coronavirus 2-specific antibody responses in coronavirus disease 2019 patients. Emerg Infect Dis. 2020;26(7). https://doi.org/10.3201/eid2607.200841
Jin Y, Wang M, Zuo Z, et al. Diagnostic value and dynamic variance of serum antibody in coronavirus disease 2019. Int J Infect Dis. 2020;94:49-52.
Zhao J, Yuan Q, Wang H, et al. Antibody responses to SARS-CoV-2 in patients of novel coronavirus disease 2019. Clin Infect Dis. 2020. https://doi.org/10.1093/cid/ciaa344
Kabesch M, Roth S, Brandstetter S, et al. Successful containment of COVID-19 outbreack in a large maternity and perinatal center while continuing clinical service. Ped Allergy Immunol. 2020. https://doi.org/10.1111/pai.13265
Robert-Koch-Institute, Berlin, Germany. Strategies for managing contact persons in health care workers. https://www.rki.de/DE/Content/InfAZ/N/Neuartiges_Coronavirus/HCW.html. Accessed April 16, 2020.
Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3). https://doi.org/10.2807/1560-7917.ES.2020.25.3.200004
Wölfel R, Corman VM, Guggemos W, et al. Virological assessment of hospitalized patients with COVID-2019. Nature. 2020. https://doi.org/10.1038/s41586-020-2196-x