High-efficiency expression of a superior β-mannanase engineered by cooperative substitution method in Pichia pastoris and its application in preparation of prebiotic mannooligosaccharides.
Cooperative substitution
High-efficiency expression
Prebiotic mannooligosaccharides
β-mannanase
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
11
03
2020
revised:
02
05
2020
accepted:
04
05
2020
pubmed:
18
5
2020
medline:
10
6
2020
entrez:
17
5
2020
Statut:
ppublish
Résumé
β-mannanase with high specific activity is a prerequisite for the industrial preparation of prebiotic mannooligosaccharides. Three mutants, namely MEI, MER, and MEIR, were constructed by cooperative substitution based on three predominant single-point site mutations (K291E, L211I, and Q112R, respectively). Heterologous expression was facilitated in Pichia pastoris and the recombinase was characterized completely. The specific activities of MER (7481.9 U mg
Identifiants
pubmed: 32416491
pii: S0960-8524(20)30754-9
doi: 10.1016/j.biortech.2020.123482
pii:
doi:
Substances chimiques
Prebiotics
0
beta-Mannosidase
EC 3.2.1.25
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123482Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.