LDB1 and the SWI/SNF complex participate in both transcriptional activation and repression by Caenorhabditis elegans BLIMP1/PRDM1.
Animals
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ genetics
Cell Line, Tumor
Chromosomal Proteins, Non-Histone
/ metabolism
DNA-Binding Proteins
/ metabolism
Enhancer Elements, Genetic
Gene Expression Regulation
Genes, Reporter
Humans
LIM Domain Proteins
/ metabolism
Positive Regulatory Domain I-Binding Factor 1
/ metabolism
RNA Interference
Transcription Factors
/ metabolism
Transcriptional Activation
BLIMP1
Caenorhabditis elegans
PRDM1
Transcriptional regulation
Journal
Biochimica et biophysica acta. Gene regulatory mechanisms
ISSN: 1876-4320
Titre abrégé: Biochim Biophys Acta Gene Regul Mech
Pays: Netherlands
ID NLM: 101731723
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
10
01
2020
revised:
29
04
2020
accepted:
04
05
2020
pubmed:
18
5
2020
medline:
15
12
2020
entrez:
18
5
2020
Statut:
ppublish
Résumé
Transcription factors of the BLIMP1/PRDM1 family are important regulators of development. BLIMP1/PRDM1 can both activate and repress gene expression, however, the mechanism of activation is not well understood. Therefore, we looked for factors involved in gene activation by C. elegans BLMP-1, the ortholog of BLIMP1/PRDM1. BLMP-1 activates the expression of bed-3, a gene involved in vulval development. By screening nuclear proteins that function in vulval development, we identified two proteins (LDB-1 and HAM-3) required for BLMP-1 dependent bed-3 expression. LDB-1 is the sole C. elegans member of the LIM Binding Protein (LDB) family, whereas HAM-3 is an accessory subunit of the SWI/SNF complex (ortholog of human SMARCD3/BAF60C). A core SWI/SNF subunit SWSN-1 (ortholog of human SMARCC1/BAF155) is also involved. We found that LDB-1 and HAM-3 bind to BLMP-1, suggesting that BLMP-1 recruits LDB-1 and the SWI/SNF complex to activate bed-3 expression. Interestingly, LDB-1 and HAM-3 are involved in both transcriptional activation and repression. In particular, BLMP-1, LDB-1 and HAM-3 co-regulate a set of hypodermal genes including bed-3 (activated), col-124 (activated) and lin-29 (repressed). On the other hand, LDB-1 and HAM-3 are not required for activation or repression of some genes regulated by BLMP-1 (e.g. T09D3.8, nas-10). We also found that human LDB1, SMARCD3/BAF60C and SMARCC1/BAF155 all physically interact with human BLIMP1/PRDM1 in vitro and are closely associated with BLIMP1/PRDM1 in vivo. Taken together, these results identify LDB1 and SWI/SNF as likely conserved cofactors of BLIMP1/PRDM1, which participate in activation and repression of a subset of BLIMP1/PRDM1-regulated genes.
Identifiants
pubmed: 32417234
pii: S1874-9399(20)30160-7
doi: 10.1016/j.bbagrm.2020.194577
pii:
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Chromosomal Proteins, Non-Histone
0
DNA-Binding Proteins
0
LDB1 protein, human
0
LIM Domain Proteins
0
Transcription Factors
0
PRDM1 protein, human
138415-26-6
Positive Regulatory Domain I-Binding Factor 1
EC 2.1.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
194577Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.