Production, characterization, and functions of sulfated polysaccharides from zinc sulfate enriched cultivation of Antrodia cinnamomea.
Animals
Antineoplastic Agents
/ chemistry
Cell Line, Tumor
Extracellular Signal-Regulated MAP Kinases
/ metabolism
Focal Adhesion Protein-Tyrosine Kinases
/ metabolism
Fungal Polysaccharides
/ chemistry
Humans
MAP Kinase Signaling System
/ drug effects
Mice
NF-kappa B
/ metabolism
Polyporales
/ chemistry
RAW 264.7 Cells
Receptors, Transforming Growth Factor beta
/ metabolism
Snail Family Transcription Factors
/ metabolism
Zinc Sulfate
/ metabolism
p38 Mitogen-Activated Protein Kinases
/ metabolism
Anti-cancer
Anti-inflammation
Antrodia cinnamomea
Sulfated polysaccharides
Zinc sulfate
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Sep 2020
15 Sep 2020
Historique:
received:
25
02
2020
revised:
07
05
2020
accepted:
10
05
2020
pubmed:
18
5
2020
medline:
16
3
2021
entrez:
18
5
2020
Statut:
ppublish
Résumé
This research utilized zinc sulfate enriched cultural conditions to produce sulfated polysaccharides from Antrodia cinnamomea (denoted as ZnFSPS) and physiochemically characterize functional and mechanical investigations of ZnFSPS. The maximum SPS yield reached a value of 6.68% when A. cinnamomea was fed zinc sulfate with 250 mM (denoted as Zn250). Zn250 had a maximal inhibitory effect on LPS-induced tumor necrosis factor (TNF-α) release in RAW264.7 macrophage. Zn250 contained the highest area percentage of molecular weight of 178.5, 105.1, and 1.56 kDa at values of 19.08, 15.09, and 5.04. Zn250 contained three times the sulfate content as compared with the control. Mechanism studies revealed a novel finding that Zn250 inhibited the LPS-induced RAW264.7 macrophage inflammation and selectively blocked pAKT, pERK and p38. Zn250 also attenuated the LPS-induced IkB-α degradation. In addition, ZnFSPS interfered with lung cancer cell H1975 TGFRI/FAK/Slug signaling. These results suggest ZnFSPS plays roles in regulating inflammatory and anti-lung cancer activity.
Identifiants
pubmed: 32417542
pii: S0141-8130(20)33216-5
doi: 10.1016/j.ijbiomac.2020.05.068
pii:
doi:
Substances chimiques
Antineoplastic Agents
0
Fungal Polysaccharides
0
NF-kappa B
0
Receptors, Transforming Growth Factor beta
0
SNAI1 protein, human
0
Snail Family Transcription Factors
0
Zinc Sulfate
7733-02-0
Focal Adhesion Protein-Tyrosine Kinases
EC 2.7.10.2
Extracellular Signal-Regulated MAP Kinases
EC 2.7.11.24
p38 Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1013-1021Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.