Physiological and metabolic features of mice with CRISPR/Cas9-mediated loss-of-function in growth hormone-releasing hormone.
Absorptiometry, Photon
Animals
Body Composition
/ genetics
Body Weight
/ genetics
CRISPR-Associated Protein 9
/ genetics
Calorimetry, Indirect
Energy Metabolism
/ genetics
Female
Growth Hormone
/ deficiency
Growth Hormone-Releasing Hormone
/ genetics
Insulin Resistance
/ genetics
Longevity
/ genetics
Loss of Function Mutation
/ physiology
Male
Mice
CRISPR
GHRH
aging
lifespan
metabolism
Journal
Aging
ISSN: 1945-4589
Titre abrégé: Aging (Albany NY)
Pays: United States
ID NLM: 101508617
Informations de publication
Date de publication:
18 05 2020
18 05 2020
Historique:
received:
08
02
2020
accepted:
20
04
2020
pubmed:
19
5
2020
medline:
20
2
2021
entrez:
19
5
2020
Statut:
ppublish
Résumé
Our previous study demonstrated that the loss of growth hormone releasing hormone (GHRH) results in increased lifespan and improved metabolic homeostasis in the mouse model generated by classical embryonic stem cell-based gene-targeting method. In this study, we targeted the GHRH gene using the CRISPR/Cas9 technology to avoid passenger alleles/mutations and performed in-depth physiological and metabolic characterization. In agreement with our previous observations, male and female GHRH
Identifiants
pubmed: 32422607
doi: 10.18632/aging.103242
pii: 103242
pmc: PMC7288930
doi:
Substances chimiques
Growth Hormone
9002-72-6
Growth Hormone-Releasing Hormone
9034-39-3
CRISPR-Associated Protein 9
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
9761-9780Subventions
Organisme : NIA NIH HHS
ID : K01 AG048264
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK079626
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG057734
Pays : United States
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