Fam20C regulates protein secretion by Cab45 phosphorylation.
CRISPR-Cas Systems
Calcium-Binding Proteins
/ genetics
Casein Kinase I
/ deficiency
Cell Line, Tumor
Extracellular Matrix Proteins
/ deficiency
Gene Knockout Techniques
Glycoproteins
/ genetics
Humans
Isoantigens
/ metabolism
Mutation
Phosphorylation
Protein Transport
/ genetics
RNA, Small Interfering
Recombinant Proteins
Seminal Plasma Proteins
/ metabolism
trans-Golgi Network
/ metabolism
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 06 2020
01 06 2020
Historique:
received:
14
10
2019
revised:
06
03
2020
accepted:
02
04
2020
entrez:
19
5
2020
pubmed:
19
5
2020
medline:
20
3
2021
Statut:
ppublish
Résumé
The TGN is a key compartment for the sorting and secretion of newly synthesized proteins. At the TGN, soluble proteins are sorted based on the instructions carried in their oligosaccharide backbones or by a Ca2+-mediated process that involves the cargo-sorting protein Cab45. Here, we show that Cab45 is phosphorylated by the Golgi-specific protein kinase Fam20C. Mimicking of phosphorylation translocates Cab45 into TGN-derived vesicles, which goes along with an increased export of LyzC, a Cab45 client. Our findings demonstrate that Fam20C plays a key role in the export of Cab45 clients by fine-tuning Cab45 oligomerization and thus impacts Cab45 retention in the TGN.
Identifiants
pubmed: 32422653
pii: 151783
doi: 10.1083/jcb.201910089
pmc: PMC7265331
pii:
doi:
Substances chimiques
Calcium-Binding Proteins
0
Extracellular Matrix Proteins
0
Glycoproteins
0
Isoantigens
0
RNA, Small Interfering
0
Recombinant Proteins
0
SPACA3 protein, human
0
Seminal Plasma Proteins
0
SDF4 protein, human
176592-21-5
Casein Kinase I
EC 2.7.11.1
FAM20C protein, human
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM134083
Pays : United States
Informations de copyright
© 2020 Hecht et al.
Références
Biochemistry. 1998 Feb 17;37(7):1789-99
pubmed: 9485304
EMBO J. 2012 Oct 17;31(20):3976-90
pubmed: 22909819
Nat Rev Microbiol. 2010 Feb;8(2):105-16
pubmed: 20023663
Trends Cell Biol. 2014 Jan;24(1):3-5
pubmed: 24373306
J Proteome Res. 2013 Jan 4;12(1):260-71
pubmed: 23186163
Commun Integr Biol. 2010 Sep;3(5):462-4
pubmed: 21057641
Nat Biotechnol. 2015 Sep;33(9):990-5
pubmed: 26280412
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
FEBS Lett. 2019 Sep;593(17):2412-2427
pubmed: 31344259
J Cell Biol. 1990 Sep;111(3):893-9
pubmed: 2167898
Traffic. 2004 May;5(5):315-26
pubmed: 15086781
Biochim Biophys Acta. 2009 Apr;1793(4):605-14
pubmed: 19046998
J Cell Biol. 1996 Apr;133(2):257-68
pubmed: 8609160
Cell. 1989 Nov 17;59(4):729-37
pubmed: 2510935
Curr Opin Cell Biol. 2008 Apr;20(2):208-13
pubmed: 18282697
Proc Natl Acad Sci U S A. 2016 Jun 14;113(24):6677-82
pubmed: 27247384
FEBS Lett. 2019 Sep;593(17):2452-2465
pubmed: 31344261
J Cell Biol. 2001 Feb 5;152(3):595-606
pubmed: 11157985
J Cell Biol. 1998 Jun 29;141(7):1515-27
pubmed: 9647645
Nature. 2016 May 25;534(7605):55-62
pubmed: 27251275
J Cell Biol. 2004 Nov 22;167(4):699-709
pubmed: 15557121
Dev Cell. 2011 May 17;20(5):652-62
pubmed: 21571222
Nature. 2007 Nov 29;450(7170):670-5
pubmed: 18046403
Cold Spring Harb Perspect Biol. 2013 Nov 01;5(11):a016790
pubmed: 24186068
Trends Cell Biol. 2014 Oct;24(10):584-93
pubmed: 24841758
Trends Cell Biol. 2005 Apr;15(4):222-8
pubmed: 15817379
Nat Methods. 2012 Mar 11;9(5):493-8
pubmed: 22406856
Nat Rev Mol Cell Biol. 2008 Nov;9(11):833-45
pubmed: 18946473
Nat Methods. 2014 Mar;11(3):319-24
pubmed: 24487582
J Cell Biol. 2009 Dec 28;187(7):1055-69
pubmed: 20026655
FEBS Lett. 1993 Sep 6;330(1):1-4
pubmed: 8370450
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Cold Spring Harb Perspect Biol. 2011 Jul 01;3(7):
pubmed: 21502307
Biochem Biophys Res Commun. 1994 Mar 30;199(3):1388-93
pubmed: 8147883
Trends Biochem Sci. 2013 Mar;38(3):121-30
pubmed: 23276407
Cell. 1999 Oct 15;99(2):189-98
pubmed: 10535737
Biochim Biophys Acta. 1980 Sep 30;604(2):129-58
pubmed: 7000188
Nat Rev Mol Cell Biol. 2008 Apr;9(4):273-84
pubmed: 18354421
Dev Cell. 2018 Nov 19;47(4):464-478.e8
pubmed: 30393074
Nat Rev Mol Cell Biol. 2004 Feb;5(2):110-20
pubmed: 15040444
Annu Rev Cell Dev Biol. 2014;30:169-206
pubmed: 25150009
Mol Cell Biol. 2014 Mar;34(5):778-93
pubmed: 24344201
ACS Nano. 2017 Jan 24;11(1):314-324
pubmed: 27943675
Curr Opin Cell Biol. 2005 Aug;17(4):395-401
pubmed: 15975778
J Cell Biol. 2012 Dec 24;199(7):1057-66
pubmed: 23266954
Mol Biol Cell. 2018 Feb 1;29(3):235-240
pubmed: 29382805
Essays Biochem. 2012;53:29-39
pubmed: 22928506
J Cell Biol. 2016 May 9;213(3):305-14
pubmed: 27138253
J Biol Chem. 2001 Feb 16;276(7):5346-52
pubmed: 11096101
Science. 2012 Jun 1;336(6085):1150-3
pubmed: 22582013
EMBO J. 1995 Oct 2;14(19):4695-704
pubmed: 7588599
J Cell Biol. 2014 Sep 1;206(5):635-54
pubmed: 25179631
J Cell Biol. 1999 Apr 19;145(2):279-89
pubmed: 10209024
Eur J Cell Biol. 2017 Aug;96(5):383-390
pubmed: 28372832
Cell. 2015 Jun 18;161(7):1619-32
pubmed: 26091039
Nat Protoc. 2013 Nov;8(11):2281-2308
pubmed: 24157548
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5004-9
pubmed: 23476064
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
Anal Chem. 2003 Feb 1;75(3):663-70
pubmed: 12585499