Sepsis Triggers a Late Expansion of Functionally Impaired Tissue-Vascular Inflammatory Monocytes During Clinical Recovery.
lung
monocytes
phagocytosis
secondary infection
sepsis
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
09
12
2019
accepted:
25
03
2020
entrez:
20
5
2020
pubmed:
20
5
2020
medline:
27
3
2021
Statut:
epublish
Résumé
Sepsis is characterized by a systemic inflammation that can cause an immune dysfunction, for which the underlying mechanisms are unclear. We investigated the impact of cecal ligature and puncture (CLP)-mediated polymicrobial sepsis on monocyte (Mo) mobilization and functions. Our results show that CLP led to two consecutive phases of Mo deployment. The first one occurred within the first 3 days after the induction of the peritonitis, while the second phase was of a larger amplitude and extended up to a month after apparent clinical recovery. The latter was associated with the expansion of Mo in the tissue reservoirs (bone marrow and spleen), their release in the blood and their accumulation in the vasculature of peripheral non-lymphoid tissues. It occurred even after antibiotic treatment but relied on inflammatory-dependent pathways and inversely correlated with increased susceptibility and severity to a secondary infection. The intravascular lung Mo displayed limited activation capacity, impaired phagocytic functions and failed to transfer efficient protection against a secondary infection into monocytopenic CCR2-deficient mice. In conclusion, our work unveiled key dysfunctions of intravascular inflammatory Mo during the recovery phase of sepsis and provided new insights to improve patient protection against secondary infections.
Identifiants
pubmed: 32425929
doi: 10.3389/fimmu.2020.00675
pmc: PMC7212400
doi:
Substances chimiques
Antigens, Ly
0
CX3C Chemokine Receptor 1
0
Ccr2 protein, mouse
0
Cx3cr1 protein, mouse
0
Ly-6C antigen, mouse
0
Receptors, CCR2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
675Informations de copyright
Copyright © 2020 Baudesson de Chanville, Chousterman, Hamon, Laviron, Guillou, Loyher, Meghraoui-Kheddar, Barthelemy, Deterre, Boissonnas and Combadière.
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