DNA vaccine protection against SARS-CoV-2 in rhesus macaques.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
14 08 2020
Historique:
received: 05 05 2020
accepted: 16 05 2020
pubmed: 22 5 2020
medline: 28 8 2020
entrez: 22 5 2020
Statut: ppublish

Résumé

The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made the development of a vaccine a top biomedical priority. In this study, we developed a series of DNA vaccine candidates expressing different forms of the SARS-CoV-2 spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals developed humoral and cellular immune responses, including neutralizing antibody titers at levels comparable to those found in convalescent humans and macaques infected with SARS-CoV-2. After vaccination, all animals were challenged with SARS-CoV-2, and the vaccine encoding the full-length S protein resulted in >3.1 and >3.7 log

Identifiants

pubmed: 32434945
pii: science.abc6284
doi: 10.1126/science.abc6284
pmc: PMC7243363
doi:

Substances chimiques

Adjuvants, Immunologic 0
Antibodies, Neutralizing 0
Antibodies, Viral 0
COVID-19 Vaccines 0
Mutant Proteins 0
Spike Glycoprotein, Coronavirus 0
Vaccines, DNA 0
Viral Vaccines 0
spike protein, SARS-CoV-2 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

806-811

Subventions

Organisme : NIAID NIH HHS
ID : T32 AI007387
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI110700
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007151
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI139538
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI108197
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI126603
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI146779
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI121394
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI149644
Pays : United States
Organisme : NIH HHS
ID : R01 OD024917
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI124377
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI100625
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI129797
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI132178
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI128751
Pays : United States

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

Références

N Engl J Med. 2020 Mar 26;382(13):1199-1207
pubmed: 31995857
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12995-3000
pubmed: 14569023
Antiviral Res. 2018 Feb;150:30-38
pubmed: 29246504
Science. 2020 May 29;368(6494):945-946
pubmed: 32385100
Nature. 2004 Apr 1;428(6982):561-4
pubmed: 15024391
Lancet. 2020 Feb 15;395(10223):507-513
pubmed: 32007143
Cell. 2015 Nov 5;163(4):988-98
pubmed: 26544943
Vaccine. 2008 Nov 25;26(50):6338-43
pubmed: 18824060
Nature. 2020 Mar;579(7798):265-269
pubmed: 32015508
Lancet. 2020 Feb 15;395(10223):514-523
pubmed: 31986261
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357
pubmed: 28807998
Vaccine. 2007 Jul 9;25(28):5220-31
pubmed: 17559989
Sci Transl Med. 2018 Jul 18;10(450):
pubmed: 30021887
Science. 2020 Jul 3;369(6499):77-81
pubmed: 32376603
Vaccine. 2005 May 2;23(24):3202-9
pubmed: 15837221
JCI Insight. 2019 Feb 21;4(4):
pubmed: 30830861
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
Nature. 2016 Mar 3;531(7592):118-21
pubmed: 26935699
N Engl J Med. 2020 Mar 5;382(10):929-936
pubmed: 32004427
Science. 2019 Sep 6;365(6457):1029-1033
pubmed: 31488689
Proc Natl Acad Sci U S A. 2013 Oct 1;110(40):16157-62
pubmed: 24043791
Science. 2020 May 29;368(6494):948-950
pubmed: 32393526
Lancet. 2020 Feb 15;395(10223):497-506
pubmed: 31986264
PLoS One. 2012;7(4):e35421
pubmed: 22536382
Science. 2020 Aug 14;369(6505):812-817
pubmed: 32434946
Sci Transl Med. 2015 Aug 19;7(301):301ra132
pubmed: 26290414

Auteurs

Jingyou Yu (J)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Lisa H Tostanoski (LH)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Lauren Peter (L)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Noe B Mercado (NB)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Katherine McMahan (K)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Shant H Mahrokhian (SH)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Joseph P Nkolola (JP)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Jinyan Liu (J)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Zhenfeng Li (Z)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Abishek Chandrashekar (A)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

David R Martinez (DR)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Carolin Loos (C)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

Caroline Atyeo (C)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

Stephanie Fischinger (S)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

John S Burke (JS)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

Matthew D Slein (MD)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

Yuezhou Chen (Y)

Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Adam Zuiani (A)

Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Felipe J N Lelis (FJN)

Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Meghan Travers (M)

Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Shaghayegh Habibi (S)

Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Laurent Pessaint (L)

Bioqual, Rockville, MD 20852, USA.

Alex Van Ry (A)

Bioqual, Rockville, MD 20852, USA.

Kelvin Blade (K)

Bioqual, Rockville, MD 20852, USA.

Renita Brown (R)

Bioqual, Rockville, MD 20852, USA.

Anthony Cook (A)

Bioqual, Rockville, MD 20852, USA.

Brad Finneyfrock (B)

Bioqual, Rockville, MD 20852, USA.

Alan Dodson (A)

Bioqual, Rockville, MD 20852, USA.

Elyse Teow (E)

Bioqual, Rockville, MD 20852, USA.

Jason Velasco (J)

Bioqual, Rockville, MD 20852, USA.

Roland Zahn (R)

Janssen Vaccines & Prevention BV, Leiden, Netherlands.

Frank Wegmann (F)

Janssen Vaccines & Prevention BV, Leiden, Netherlands.

Esther A Bondzie (EA)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Gabriel Dagotto (G)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Makda S Gebre (MS)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Xuan He (X)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Catherine Jacob-Dolan (C)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Marinela Kirilova (M)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Nicole Kordana (N)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Zijin Lin (Z)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Lori F Maxfield (LF)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Felix Nampanya (F)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Ramya Nityanandam (R)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

John D Ventura (JD)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Huahua Wan (H)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.

Yongfei Cai (Y)

Children's Hospital, Boston, MA 02115, USA.

Bing Chen (B)

Children's Hospital, Boston, MA 02115, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02215, USA.

Aaron G Schmidt (AG)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02215, USA.

Duane R Wesemann (DR)

Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02215, USA.

Ralph S Baric (RS)

Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Galit Alter (G)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02215, USA.

Hanne Andersen (H)

Bioqual, Rockville, MD 20852, USA.

Mark G Lewis (MG)

Bioqual, Rockville, MD 20852, USA.

Dan H Barouch (DH)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA. dbarouch@bidmc.harvard.edu.
Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02215, USA.

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