Morphokinetic analysis of cleavage stage embryos and assessment of specific gene expression in cumulus cells independently predict human embryo development to expanded blastocyst: a preliminary study.
Adult
Blastocyst
/ metabolism
Cleavage Stage, Ovum
/ metabolism
Cumulus Cells
/ metabolism
Embryo Culture Techniques
Embryo Implantation
/ genetics
Embryo Transfer
/ methods
Embryo, Mammalian
Embryonic Development
/ genetics
Female
Gene Expression Regulation, Developmental
/ genetics
Humans
Time-Lapse Imaging
Blastocyst
Cumulus cells
Embryo morphology
Gene expression
Human IVF
Time-lapse
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
09
12
2019
accepted:
01
05
2020
pubmed:
22
5
2020
medline:
4
2
2021
entrez:
22
5
2020
Statut:
ppublish
Résumé
To assess whether morphokinetic features at the cleavage stage together with specific gene expression in cumulus cells (CCs) may be used to predict whether human embryos are able to achieve the expanded blastocyst stage on day 5. Eighty-one embryos were cultured using the Geri plus® time-lapse system. Twenty-seven embryos progressing to the expanded blastocyst stage (BL group) were compared with thirty-five embryos showing developmental arrest (AR group) and nineteen reaching the stage of early or not fully expanded blastocyst (nBL group). The analyzed morphokinetic variables were pronuclear appearance (tPNa), pronuclear fading (tPNf), and completion of cleavage to two, three, four, and eight cells (t2, t3, t4, and t8). CCs were analyzed by RT-qPCR for bone morphogenetic protein 15 (BMP15), cytochrome c oxidase subunit II (COXII), ATP synthase subunit 6 (MT-ATP6), connexin 43 (Cx43), and heme oxygenase-1 (HO-1). Embryos of BL group showed a significantly faster kinetic. BMP15, COXII, and MT-ATP6 mRNA expression was significantly higher in CCs of BL group embryos, whereas Cx43 and HO-1 mRNA levels were higher in AR group. Kinetic parameters and gene expression were not significantly different between either the BL and nBL groups or the AR and nBL groups. ROC curves showed that the most predictive cut-offs were t2 < 26.25 for morphokinetics and COXII > 0.3 for gene expression. Multivariable logistic regression analysis showed that morphokinetic variables and gene expression were both valuable, independent predictors of embryo development to expanded blastocyst. Our results suggest the possibility of developing integrated prediction models for early embryo selection at the cleavage stage.
Identifiants
pubmed: 32436046
doi: 10.1007/s10815-020-01806-6
pii: 10.1007/s10815-020-01806-6
pmc: PMC7311629
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1409-1420Références
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