Simultaneous quantification of oestrogens and androgens in the serum of patients with benign prostatic hyperplasia by liquid chromatography-Tandem mass spectrometry.
LC-MS/MS
androgens
benign prostate hyperplasia
clinical samples
hormones homeostasis
oestrogens
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
15
02
2020
revised:
29
03
2020
accepted:
01
04
2020
pubmed:
23
5
2020
medline:
22
6
2021
entrez:
23
5
2020
Statut:
ppublish
Résumé
Benign prostate hyperplasia (BPH) is a common disease in elderly men. It has been found that the occurrence of BPH was closely related to dysregulated steroid hormones. Here, a rapid, sensitive, accurate and specific method for the quantitative profiling of five androgens in man serum was developed and validated by the use of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Using this method, dehydroepiandrosterone (DHEA), androstenedione (A4), testosterone (T), androsterone (A), dihydrotestosterone (DHT), oestrone (E1) and oestradiol (E2) were quantified in serum from man with and without BPH. BPH patients were characterised by the decreases in DHEA, A4 and T as well as increases in DHT, E2 and E1 in serum. Meanwhile, DHEA and DHT in serum were screened as sensitive biomarkers of BPH patients. This study will provide a new perspective of dysregulated steroid hormones for the diagnosis and prevention of BPH.
Substances chimiques
Androgens
0
Estrogens
0
Dihydrotestosterone
08J2K08A3Y
Testosterone
3XMK78S47O
Androstenedione
409J2J96VR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13611Subventions
Organisme : the initializing Fund of Xuzhou Medical University
ID : 53681720
Organisme : National Natural Science Foundation of China
ID : 81903689
Organisme : National Natural Science Foundation of China
ID : 81973377
Organisme : Key Natural Science Foundation of Jiangsu Higher Education Institutions of China
ID : 19KJA460008
Organisme : Key Natural Science Foundation of Jiangsu Higher Education Institutions of China
ID : 19KJB350006
Organisme : Foundation of Jiangsu Province
ID : BRA2015329
Organisme : Jiangsu Overseas Research & Training Program for University Young & Middle-aged Teachers and Presidents
Organisme : Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Informations de copyright
© 2020 Blackwell Verlag GmbH.
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