IgG Fc sialylation is regulated during the germinal center reaction following immunization with different adjuvants.
Adjuvants, Immunologic
/ administration & dosage
Alum Compounds
/ administration & dosage
Animals
Antigens
/ administration & dosage
B-Lymphocytes
/ drug effects
Cytokines
/ immunology
Female
Freund's Adjuvant
/ administration & dosage
Germinal Center
/ immunology
Glycosylation
Immunoglobulin G
/ immunology
Lipopolysaccharides
/ administration & dosage
Mice, Inbred C57BL
Mice, Knockout
Mineral Oil
/ administration & dosage
Mycobacterium tuberculosis
/ immunology
Ovalbumin
/ administration & dosage
Polysorbates
/ administration & dosage
Squalene
/ administration & dosage
T-Lymphocytes
/ drug effects
Vaccination
Antibodies
IFN-γ
IL-17
IL-27R
IL-6
IgG glycosylation
T follicular cells
adjuvants
germinal center
vaccination
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
12
12
2019
revised:
23
04
2020
accepted:
24
04
2020
pubmed:
24
5
2020
medline:
11
3
2021
entrez:
24
5
2020
Statut:
ppublish
Résumé
Effector functions of IgG Abs are regulated by their Fc N-glycosylation pattern. IgG Fc glycans that lack galactose and terminal sialic acid residues correlate with the severity of inflammatory (auto)immune disorders and have also been linked to protection against viral infection and discussed in the context of vaccine-induced protection. In contrast, sialylated IgG Abs have shown immunosuppressive effects. We sought to investigate IgG glycosylation programming during the germinal center (GC) reaction following immunization of mice with a foreign protein antigen and different adjuvants. Mice were analyzed for GC T-cell, B-cell, and plasma cell responses, as well as for antigen-specific serum IgG subclass titers and Fc glycosylation patterns. Different adjuvants induce distinct IgG This study's findings regarding adjuvant-dependent GC responses and IgG glycosylation programming may aid in the development of novel vaccination strategies to induce IgG Abs with both high affinity and defined Fc glycosylation patterns in the GC.
Sections du résumé
BACKGROUND
Effector functions of IgG Abs are regulated by their Fc N-glycosylation pattern. IgG Fc glycans that lack galactose and terminal sialic acid residues correlate with the severity of inflammatory (auto)immune disorders and have also been linked to protection against viral infection and discussed in the context of vaccine-induced protection. In contrast, sialylated IgG Abs have shown immunosuppressive effects.
OBJECTIVE
We sought to investigate IgG glycosylation programming during the germinal center (GC) reaction following immunization of mice with a foreign protein antigen and different adjuvants.
METHODS
Mice were analyzed for GC T-cell, B-cell, and plasma cell responses, as well as for antigen-specific serum IgG subclass titers and Fc glycosylation patterns.
RESULTS
Different adjuvants induce distinct IgG
CONCLUSION
This study's findings regarding adjuvant-dependent GC responses and IgG glycosylation programming may aid in the development of novel vaccination strategies to induce IgG Abs with both high affinity and defined Fc glycosylation patterns in the GC.
Identifiants
pubmed: 32445838
pii: S0091-6749(20)30728-4
doi: 10.1016/j.jaci.2020.04.059
pii:
doi:
Substances chimiques
Addavax
0
Adjuvants, Immunologic
0
Alum Compounds
0
Antigens
0
Cytokines
0
Immunoglobulin G
0
Lipopolysaccharides
0
Monatide (IMS 3015)
0
Polysorbates
0
aluminum sulfate
34S289N54E
Squalene
7QWM220FJH
Mineral Oil
8020-83-5
Ovalbumin
9006-59-1
Freund's Adjuvant
9007-81-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
652-666.e11Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.