Proteins of Wnt signaling pathway in cancer stem cells of human glioblastoma.
Cancer stem cells
Glioblastoma
Wnt/β-catenin signaling pathway
Journal
International review of neurobiology
ISSN: 2162-5514
Titre abrégé: Int Rev Neurobiol
Pays: United States
ID NLM: 0374740
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
26
5
2020
pubmed:
26
5
2020
medline:
4
6
2021
Statut:
ppublish
Résumé
Glioblastoma multiforme (GBM) is the most aggressive primary glial brain tumor. The prognosis for GBM patients is not favorable, with the median survival time being 15 months. Its treatment resistance is associated with GBM cell population having cancer stem cells (CSCs). Wnt/β-catenin signaling pathway is a strategically important molecular mechanism, providing proliferation of stem cells of all types. This study compares the expression levels of signaling pathway proteins in CD133(+) CSCs and CD133(-) differentiated glioblastoma cells (DGCs). the present study used U-87MG cells of human glioblastoma, the material was tested for mycoplasma contamination. High-performance liquid chromatography (HPLC) mass spectrometry was used for proteome analysis. Biological and molecular functions, signaling pathways and protein-protein interactions were analyzed using free-access databases: PubMed, PANTHER, Gene Ontology, Swiss-Prot and KEGG. Protein-protein interactions (PPIs) were analyzed using the STRING database (version 10). There were identified 589 proteins with significantly changed expression in CD133+ CSCs, as compared with CD133-DGCs (P<0.05). Bioinformatics analysis allowed to attribute 134 differentially expressed proteins to 16 signaling pathways. A significant increase in expression of eight Wnt signaling pathway proteins (APC, CSNK1E, CSNK1A, CSNK2A2, CSNK2B, CTNNB1, DVL1, RUVBL) was detected, as well as four proteins of the non-canonical Wnt pathway-RHOA, ROCK2, RAC2, DAAM1. Special attention should be paid to β-catenin (CTNNB1) with more than 13.98-fold increase of expression in CSCs and Disheveled-associated activator of morphogenesis 1 (DAAM1) with 6.15-fold higher upregulation level. proteins of Wnt/β-catenin signaling cascade are a prospective target for regulating CSCs activity.
Identifiants
pubmed: 32448607
pii: S0074-7742(20)30027-1
doi: 10.1016/bs.irn.2020.03.006
pii:
doi:
Substances chimiques
AC133 Antigen
0
PROM1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
185-200Informations de copyright
© 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare there are no conflicts of interests.